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FnrL regulon in Rhodobacter sphaeroides

ABSTRACT: In this study, we performed a ChIP-chip experiment to determine the regulon of FnrL in Rhodobacter sphaeroides. We grew R. sphaeroides under anaerobic photosnthetic conditions, in which FnrL is expected to bind DNA and contol gene expression, and immuno-precipitated FnrL, but also sigma70 and the Beta' subunits of RNA polymerase to determine transcription activity. DNA immunoprecipitated with polyclonal antibodies against FnrL, sigma70, or the Beta' subunit of RNA polymerase was labelled with Cy5 and hybridized on two-color tilling arrays (triplicates for each) with genomic DNA as an input control labelled with Cy3.

ORGANISM(S): Rhodobacter sphaeroides 2.4.1

SUBMITTER: Yann Dufour

PROVIDER: E-GEOD-22027 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Reconstruction of the core and extended regulons of global transcription factors.

Dufour Yann S YS Kiley Patricia J PJ Donohue Timothy J TJ

PLoS genetics 20100722 7

The processes underlying the evolution of regulatory networks are unclear. To address this question, we used a comparative genomics approach that takes advantage of the large number of sequenced bacterial genomes to predict conserved and variable members of transcriptional regulatory networks across phylogenetically related organisms. Specifically, we developed a computational method to predict the conserved regulons of transcription factors across alpha-proteobacteria. We focused on the CRP/FNR ...[more]

PMID: 20661434

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