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Streptomyces coelicolor A3(2) SigR regulon

ABSTRACT: We determined the genomic locations of the SigR binding sites by chromatin immuno-precipitation of cross-linked DNA and hybridization on a tilling oligonucleotide array after 20 minutes of diamide treatment on cell cultures. Three independent biological samples were prepared separately from the wild-type cells and pooled before hybridization on a single array. Three more independent biological samples were prepared from sigR deletion mutant cells, pooled, and hybridized on another single array. After normalization, the signal from the mutant cells was subtracted from the signal from the wild-type cells to recover SigR specific signal.

ORGANISM(S): Streptomyces coelicolor A3(2)

SUBMITTER: Yann Dufour

PROVIDER: E-GEOD-33593 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Conservation of thiol-oxidative stress responses regulated by SigR orthologues in actinomycetes.

Kim Min-Sik MS Dufour Yann S YS Yoo Ji Sun JS Cho Yoo-Bok YB Park Joo-Hong JH Nam Gi-Baeg GB Kim Hae Min HM Lee Kang-Lok KL Donohue Timothy J TJ Roe Jung-Hye JH

Molecular microbiology 20120614 2

Numerous thiol-reactive compounds cause oxidative stress where cells counteract by activation of survival strategies regulated by thiol-based sensors. In Streptomyces coelicolor, a model actinomycete, a sigma/antisigma pair SigR/RsrA controls the response to thiol-oxidative stress. To unravel its full physiological functions, chromatin immuno-precipitation combined with sequence and transcript analyses were employed to identify 108 SigR target genes in S. coelicolor and to predict orthologous re ...[more]

PMID: 22651816

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