Unknown,Transcriptomics,Genomics,Proteomics

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Global Deterministic and Stochastic Allelic Specific Gene Expression in Single Blastomeres of Mouse Early Embryos


ABSTRACT: Here we modified a single cell whole transcriptome amplification method to make it capable of amplifying cDNAs as long as 3kb efficiently and unbiasedly. We combined this modified single cell cDNA amplification method with Applied Biosystems next generation sequencing SOLiD™ System to set up a single cell whole transcriptome assay. The modified amplification strategy allows us to amplify full-length cDNAs for most of the expressed genes. We show that it is feasible to get digital gene expression profiles at single cell resolution. This allows us to ask fundamental biological questions that could not be addressed previously and to understand transcriptome complexity at the resolution of a single cell. Oocyte, Two-cell, Four-cell, and 8-cell stage embryos were recovered from MF1 females mated with MF1 male mice (Nagy et al. 2003). The zona pellucida was removed by treatment with acidic tyrode solution. The individual blastomeres were separated by gentle pipeting using a glass capillary. RNA-Seq from 24 single mouse blastomeres from oocytes, 2-cell, 4-cell and 8-cell stages.

ORGANISM(S): Mus musculus

SUBMITTER: Catalin Barbacioru 

PROVIDER: E-GEOD-22182 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Deterministic and stochastic allele specific gene expression in single mouse blastomeres.

Tang Fuchou F   Barbacioru Catalin C   Nordman Ellen E   Bao Siqin S   Lee Caroline C   Wang Xiaohui X   Tuch Brian B BB   Heard Edith E   Lao Kaiqin K   Surani M Azim MA  

PloS one 20110623 6


Stochastic and deterministic allele specific gene expression (ASE) might influence single cell phenotype, but the extent and nature of the phenomenon at the onset of early mouse development is unknown. Here we performed single cell RNA-Seq analysis of single blastomeres of mouse embryos, which revealed significant changes in the transcriptome. Importantly, over half of the transcripts with detectable genetic polymorphisms exhibit ASE, most notably, individual blastomeres from the same two-cell e  ...[more]

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