Dataset Information
Label-free Quantification of Proteins in Single Embryonic Cells with Neural Fate in the Cleavage-Stage Frog (Xenopus laevis) Embryo using CE-ESI-HRMS
Ontology highlight
ABSTRACT:
INSTRUMENT(S): Bruker Daltonics maXis series, Orbitrap Fusion
ORGANISM(S): Xenopus Laevis (african Clawed Frog)
TISSUE(S): Embryo, Early Embryonic Cell
SUBMITTER: 
Camille Lombard-Banek
LAB HEAD: Peter Nemes
PROVIDER: PXD004174 | Pride | 2016-06-23
REPOSITORIES: Pride
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| 2014-10-22_CL03_Embx2dil_4Hz.d.zip | Other | |||
| 2014-10-22_CL04_Embx2dil_4Hz.d.zip | Other | |||
| 2014-10-22_CL05_Embx2dil_4Hz.d.zip | Other | |||
| 2014-10-29_CL06_XenEmb_4Hz.d.zip | Other | |||
| 2014-11-05_CL04_Embx2.d.zip | Other |
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Publications
Label-free Quantification of Proteins in Single Embryonic Cells with Neural Fate in the Cleavage-Stage Frog (Xenopus laevis) Embryo using Capillary Electrophoresis Electrospray Ionization High-Resolution Mass Spectrometry (CE-ESI-HRMS).
Molecular & cellular proteomics : MCP 20160617 8
Quantification of protein expression in single cells promises to advance a systems-level understanding of normal development. Using a bottom-up proteomic workflow and multiplexing quantification by tandem mass tags, we recently demonstrated relative quantification between single embryonic cells (blastomeres) in the frog (Xenopus laevis) embryo. In this study, we minimize derivatization steps to enhance analytical sensitivity and use label-free quantification (LFQ) for single Xenopus cells. The t ...[more]
