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Integrative analysis reveals selective 9p24.1 amplification, increased PD-1 ligand expression, and further induction via JAK2 in nodular sclerosing Hodgkin lymphoma and primary mediastinal large B-cell lymphoma

ABSTRACT: We integrate high-resolution copy number data with transcriptional profiles and identify the immunoregulatory genes, PD-L1 and PD-L2, as key targets at the 9p24.1 amplification peak in HL and MLBCL cell lines. DNA copy number alterations were defined in DLBCL and HL cell lines, normalized to healthy donors, by GISTIC analysis of Affymetrix SNP 6.0 single nucleotide polymorphism microarray data. Using this analysis, we identified 9p24 amplification to be over-represented in HL cell lines compared to DLBCL cell lines.

ORGANISM(S): Homo sapiens

SUBMITTER: Michael Green

PROVIDER: E-GEOD-22208 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Integrative analysis reveals selective 9p24.1 amplification, increased PD-1 ligand expression, and further induction via JAK2 in nodular sclerosing Hodgkin lymphoma and primary mediastinal large B-cell lymphoma.

Green Michael R MR Monti Stefano S Rodig Scott J SJ Juszczynski Przemyslaw P Currie Treeve T O'Donnell Evan E Chapuy Bjoern B Takeyama Kunihiko K Neuberg Donna D Golub Todd R TR Kutok Jeffery L JL Shipp Margaret A MA

Blood 20100713 17

Classical Hodgkin lymphoma (cHL) and mediastinal large B-cell lymphoma (MLBCL) are lymphoid malignancies with certain shared clinical, histologic, and molecular features. Primary cHLs and MLBCLs include variable numbers of malignant cells within an inflammatory infiltrate, suggesting that these tumors escape immune surveillance. Herein, we integrate high-resolution copy number data with transcriptional profiles and identify the immunoregulatory genes, PD-L1 and PD-L2, as key targets at the 9p24. ...[more]

PMID: 20628145

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