Unknown,Transcriptomics,Genomics,Proteomics

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MicroRNA-target RNA pairs revealed by Parallel analysis of RNA ends in Solanum lycopersicum

ABSTRACT: Solanum lycopersicum RNA degradome sequencing Isolated polyadenylated RNA from total RNA extracts of Solanum lycopersicum, were ligated to 5'-adapter that includes an MmeI recognition site. The ligated products were purified again, reverse transcribed and cleaved with MmeI. The 5' fragments were purified from gel and ligated to a 3'- dsDNA adapter and PCR amplified. After PCR amplification the sample was subjected to Solexa/Illumina high throughput pyrosequencing. Please see www.illumina.com for details of the sequencing technology.

ORGANISM(S): Solanum lycopersicum

SUBMITTER: Simon Moxon

PROVIDER: E-GEOD-22236 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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MicroRNA-target RNA pairs revealed by Parallel analysis of RNA ends in Solanum lycopersicum

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