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High-throughput sequencing of small RNAs in Medicago truncatula

ABSTRACT: Medicago truncatula endogenous small RNAs The dataset contains Medicago truncatula Gaertn. cv. Jemalong endogenous small RNA sequences in the range 18-28 nucleotides. High-throughput Solexa/Illumina sequencing was carried out at the Sainsbury Laboratory, Norwich, UK. Please see www.illumina.com for details of the technology. Small RNA sequences were mapped to Medicago truncatula genome release 2.0 (http://www.medicago.org/genome/), the number of matches to the unfinished genome, if any, is recorded in the Series supplementary file GSE13761_sequence_annotations.txt.gz. Size fractionated small RNA from total RNA extracts was ligated to adapters, purified again and reverse transcribed. After PCR amplification the sample was subjected to Solexa/Illumina high throughput pyrosequencing. Please see www.illumina.com for details of the sequencing technology.

ORGANISM(S): Medicago truncatula

SUBMITTER: Simon Moxon 

PROVIDER: E-GEOD-13761 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

High-throughput sequencing of Medicago truncatula short RNAs identifies eight new miRNA families.

Szittya Gyorgy G   Moxon Simon S   Santos Dulce M DM   Jing Runchun R   Fevereiro Manuel P S MP   Moulton Vincent V   Dalmay Tamas T  

BMC genomics 20081209

<h4>Background</h4>High-throughput sequencing technology is capable to identify novel short RNAs in plant species. We used Solexa sequencing to find new microRNAs in one of the model legume species, barrel medic (Medicago truncatula).<h4>Results</h4>3,948,871 reads were obtained from two separate short RNA libraries generated from total RNA extracted from M. truncatula leaves, representing 1,563,959 distinct sequences. 2,168,937 reads were mapped to the available M. truncatula genome correspondi  ...[more]

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