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UDP-GlcNAc 2-epimerase/ManNAc kinase (GNE) is an inducer of apoptotic processes in Capan-1 pancreatic carcinoma cells: GNE silencing

ABSTRACT: Early invasive growth and metastasis are features of pancreatic cancer that rely on resistance to anoikis, an apoptosis program activated upon loss of adequate matrix anchorage. Re-expression of the tumor suppressor p16 reversed anoikis resistance of pancreatic cancer cells. This conversion to an anoikis-susceptible phenotype was found to be associated with a striking loss of GNE mRNA expression, prompting us to address the role of GNE in pancreatic cancer in more detail. GNE catalyzes a rate-limiting key step of the sialic acid biosynthesis and may have additional functions in the nucleus. Pancreatic cancer cells Capan-1. Three GNE-silencing samples and three control samples.

ORGANISM(S): Homo sapiens

SUBMITTER: Wolfgang Kemmner

PROVIDER: E-GEOD-22336 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Loss of UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase (GNE) induces apoptotic processes in pancreatic carcinoma cells.

Kemmner Wolfgang W Kessel Pia P Sanchez-Ruderisch Hugo H Möller Heinz H Hinderlich Stephan S Schlag Peter M PM Detjen Katharina K

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 20111102 2

Early invasive growth and metastasis are features of pancreatic cancer that rely on its resistance to anoikis, an apoptosis program activated on loss of matrix anchorage. How anoikis is regulated is unclear. UDP-N-acetylglucosamine-2-epimerase/N-acetylmannosamine-kinase (GNE) was silenced, or p16 was overexpressed, in human pancreatic carcinoma cells. Gene expression profiling, enzymatic assays, Western blotting, and cell cycle analysis were conducted. Silencing of GNE, the key enzyme of sialic ...[more]

PMID: 22049060

Similar Datasets

UDP-GlcNAc 2-epimerase/ManNAc kinase (GNE) is an inducer of apoptotic processes in Capan-1 pancreatic carcinoma cells

Project description:This SuperSeries is composed of the following subset Series: GSE22334: Induction of apoptotic processes in Capan-1 pancreatic carcinoma cells by restoration of p16INK4a expression GSE22336: UDP-GlcNAc 2-epimerase/ManNAc kinase (GNE) is an inducer of apoptotic processes in Capan-1 pancreatic carcinoma cells: GNE silencing Refer to individual Series

2010-08-01 | E-GEOD-22337 | biostudies-arrayexpress

Induction of apoptotic processes in Capan-1 pancreatic carcinoma cells by restoration of p16INK4a expression

Project description:Early invasive growth and metastasis are features of pancreatic cancer that rely on resistance to anoikis, an apoptosis program activated upon loss of adequate matrix anchorage. Re-expression of the tumor suppressor p16 reversed anoikis resistance of pancreatic cancer cells. This conversion to an anoikis-susceptible phenotype was found to be associated with a striking loss of GNE mRNA expression, prompting us to address the role of GNE in pancreatic cancer in more detail. GNE catalyzes a rate-limiting key step of the sialic acid biosynthesis and may have additional functions in the nucleus. Pancreatic cancer cells Capan-1. Three p16-transfectants and three mock-transfectants.

2010-08-01 | E-GEOD-22334 | biostudies-arrayexpress

UDP-GlcNAc 2-epimerase/ManNAc kinase (GNE) is an inducer of apoptotic processes in Capan-1 pancreatic carcinoma cells: GNE silencing

2010-08-01 | GSE22336 | GEO

Induction of apoptotic processes in Capan-1 pancreatic carcinoma cells by restoration of p16INK4a expression

2010-08-01 | GSE22334 | GEO

Gene expression profiling of Capan-1 and Pt45P1 cells treated or not with alternatively spliced tissue factor (asTF)

Project description:The effects of alternatively spliced tissue factor (asTF) were measured by gene expression profiling of Capan-1 and Pt45P1 cells. 4 samples: Capan-1 cells +/- asTF, Pt45P1 cells +/- asTF.

2013-04-10 | E-GEOD-45928 | biostudies-arrayexpress

RNA-Seq of pancreatic cancer cell line CAPAN-2 with or without SIRT4 overexpression

Project description:We profile gene expression with or without SIRT4 overexpression in pancreatic cancer cell line CAPAN-2 to find the different genes and pathways regulated by SIRT4.

2022-05-31 | GSE201110 | GEO

Transcription profiling by array of apoptosis resistant and -susceptible Capan-1 cells

Project description:Pancreatic cancer cells are inherently highly resistant to spontaneous or chemotherapy-induced apoptosis. The human pancreatic tumor cell line Capan-1 (pRB+/p16-) was stably transfected with p16 expression constructs or control plasmids to functionally inactivate pRB. Three independent experiments were conducted

2015-02-27 | E-MTAB-3370 | biostudies-arrayexpress

Expression data from DMOS- or paeoniflorin- treated human pancreatic cancer cell line Capan-1

Project description:Paeoniflorin (PF) is an active monoterpene glycoside extracted from Paeonia lactiflora Pall. Recent studies showed that PF has anti-tumor effects in various types of cancer. However, the function of PF in pancreatic cancer (PA) is largely unexplored. Here, we showed that PF suppressed growth of PA cell lines Capan-1 and MIAPaCa-2, and profoundly sensitized these cells to X-ray irradiation. Through a microarray analysis, we identified tumor-suppressor candidate gene HTRA3 as the most increased gene upon PF treatment in Capan-1 cells. Ectopic expression of HTRA3 led to reduced cell growth and increased expression of apoptotic protein Bax, suggesting a tumor suppressive role of HTRA3 in PA cells. Together, our results provide a set of genetic and biologic proofs that PF inhibited PA cell growth by upregulation of HTRA3.

2017-09-20 | GSE97124 | GEO

HOXB7 mRNA is overexpressed in pancreatic ductal adenocarcinomas and its knockdown induces cell cycle arrest and apoptosis.

Project description:In order to determine the role of HOXB7 gene in cell lines derived from pancreatic ductal adenocarcinoma (MIA PaCa-2 e Capan-1), we performed its silencing utilizing the technique of RNA interference (RNAi). Total RNA derived from the inhibition as well as from parental cells was quantified in Bioanalyzer (Agilent,Santa Clara, CA, USA) and employed in the Agilent platform (4x44K). Proceeded with the guidelines of the One Color Microarray-Based Gene Expression Protocol (Agilent) and with the use of the Agilent Low Input Quick Amp Labeling Kit. HOXB7 knockdown elicited the modulation of several biological processes, especially in the MIA Paca-2 cell line, such as proteasomal ubiquitin-dependent catabolic process, synthesis of amines and cell cycle. Moreover, it showed induction of apoptosis and reduction in cell proliferation by flow cytometry and MTT assay, respectively. In this context, HOXB7 represents another component associated with the extensive network of molecules involved in the tumorigenesis of pancreatic cancer and could be a promising target for future biologic therapies. The procedure was performed in duplicate for both cell lines, which were sorted into treated and untreated with RNAi.

2013-07-26 | E-GEOD-46393 | biostudies-arrayexpress

Expression analysis of human pancreatic cancer cell lines (Capan-1, Panc-1, Panc-1+ve and Panc-1-ve) and pancreatic ductal cell line (HPDE)

Project description:Analysis of differentially expressing genes in whole genome wide analysis of aptamer SQ2 positive cells (Capan-1, Panc-1, Panc-1+ve) and SQ2 negative cells (Panc-1-ve and HPDE) Panc-1 +ve and Panc-1-ve cell lines were generated from Panc-1 cell line based upon its hetergenous binding to aptamer SQ2. Detailed procedure of generation of these cell lines are described in Pooja Dua, Hye Suk Kang, Seung-Mo Hong, Ming-Sound Tsao, Soyoun Kim, and Dong-ki Lee. 2012 Alkaline Phosphatase ALPPL2 is a novel pancreatic carcinoma-associated protein. Cancer Research A five chip study using total RNA recovered from Capan-1, Panc-1, Panc-1+ve, Panc-1-ve and HPDE cells. Each chip measures 45,033 genes with three 60 mer probe pairs per target.

2012-11-21 | E-GEOD-42428 | biostudies-arrayexpress

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