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Gene profiling: U87 IRE1 dominant negative cells vs. U87ctrl cells in culture

ABSTRACT: Transcriptome analysis was performed from human U87 glioblastoma cell clones: U87 IRE1.NCK DN (U87dn, IRE1 dominant negative) and U87 control (U87ctrl, empty plasmid). Cells were grown in DMEM supplemented with 10% FBS and glutamine for 16 hours in culture prior mRNA isolation and analyses U87dn cells expressing a dominant negative transgene of IRE1alpha were compared to U87ctrl cells transfected with the corresponding empty plamid to identify genes associated to tumor invasion and angiogenesis.

ORGANISM(S): Homo sapiens

SUBMITTER: Michel Moenner

PROVIDER: E-GEOD-22385 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Inositol-requiring enzyme 1alpha is a key regulator of angiogenesis and invasion in malignant glioma.

Auf Gregor G Jabouille Arnaud A Guérit Sylvaine S Pineau Raphaël R Delugin Maylis M Bouchecareilh Marion M Magnin Noël N Favereaux Alexandre A Maitre Marlène M Gaiser Timo T von Deimling Andreas A Czabanka Marcus M Vajkoczy Peter P Chevet Eric E Bikfalvi Andreas A Moenner Michel M

Proceedings of the National Academy of Sciences of the United States of America 20100811 35

Inositol-requiring enzyme 1 (IRE1) is a proximal endoplasmic reticulum (ER) stress sensor and a central mediator of the unfolded protein response. In a human glioma model, inhibition of IRE1alpha correlated with down-regulation of prevalent proangiogenic factors such as VEGF-A, IL-1beta, IL-6, and IL-8. Significant up-regulation of antiangiogenic gene transcripts was also apparent. These transcripts encode SPARC, decorin, thrombospondin-1, and other matrix proteins functionally linked to mesench ...[more]

PMID: 20702765

Similar Datasets

Gene profiling: U87 IRE1 dominant negative cells vs. U87ctrl cells in culture

Project description:Transcriptome analysis was performed from human U87 glioblastoma cell clones: U87 IRE1.NCK DN (U87dn, IRE1 dominant negative) and U87 control (U87ctrl, empty plasmid). Cells were grown in DMEM supplemented with 10% FBS and glutamine for 16 hours in culture prior mRNA isolation and analyses

2010-08-08 | GSE22385 | GEO

IRE1-dependent transcriptome remodelling upon ER stress in human glioma cells

Project description:We investigate the contribution of IRE1 signaling to the modulation of U87 glioma cells transcriptome upon various stresses. To this end, IRE1 control and IRE1 dominant negative expressing U87 glioma cells were subjected to environmental or chemical challenges and their transcriptome monitored using Affymetrix microarrays. Stress-induced transcriptome modulation in function of IRE1 proficiency/deficiency

2011-12-31 | E-GEOD-27306 | biostudies-arrayexpress

IRE1-dependent transcriptome remodelling upon ER stress in human glioma cells

2011-12-31 | GSE27306 | GEO

Gene expression profiles of U87 cells after transfection with hsa-miR-145-5p and 31-5p

Project description:We analyzed the expression profiles of hsa-miR-145-5p or hsa-miR-31-5p-targeting genes relating to invasion or migration after co-overexpression of hsa-miR-145-5p and 31-5p Gene expression profiles of U87 cells after co-transfection with hsa-miR-145-5p and 31-5p mimics, and U87 cells after transfection miR mimic negative control

2016-06-23 | E-GEOD-83643 | biostudies-arrayexpress

Expression data from cells overexpressing c19orf63 (HSS1) [U87]

Project description:U87 cell lines were stable transfected with C19ORF63 (Human hematopoietic peptide secreted-1 - HSS1). HSS1 is a truly novel protein defining a new class of secreted factors. U87 cell line overexpressing HSS1 greatly reduced their proliferation rate compared to mock-transfected cells. Microarray analysis was used to detail gene expression underlying the anti-proliferative and anti-tumorigenic effect of HSS1 in U87 cells. Exponentially growing U87 cells at growth curve day 5 were harvested for total RNA extraction and hybridization on Affimetrix microarrays. Three groups of samples were evaluated in triplicates: U87 wild-type, U87 -pcDNA3.1 mock-transfected, U87-pcDNA-HSS1. Cells were stable transfected with pcDNA3.1 empty vector or hHSS1. hHSS1-expressing cells and control cells were at confluence 40-80% when harvested. Trypan blue analysis of the number of viable cells showed a significant anti-proliferative effect in U87 cells expressing hHSS1 as compared to the control cells.

2014-12-22 | E-GEOD-61779 | biostudies-arrayexpress

G72 overexpression in U87 cells

Project description:G72 is a susceptible gene for schizophrenia. No matter genetic variation or serum levels, G72 has been recognized as a biomarker for schizophrenia. In some reports, G72 genetic variation also related with bipolar disorder. Although G72 has been studied for 15 years, the protein function is still unclear. This study aims for predicting the biological function of G72 and hope to elucidate its biological role. two independent control (U87 cells transfected with empty vector) and two experiements (U87 cells transfected with G72 overexpression plasmid).

2015-12-31 | E-GEOD-67704 | biostudies-arrayexpress

Comparison of gene expression levels in parental U87 cells and U87 tumor spheres treated with or without ITE

Project description:The formation of U87 tumor spheres was associated with expression changes of many genes which was reverted by the treatment with ITE(2-(1'H-indole-3'-carbonyl)-thiazole-4-carboxylic acid methyl ester). We used DNA microarrays to profile gene expression in U87 tumor spheres treated with ITE and identified distinct classes of up-regulated and down-regulated genes during this process. U87 cells were selected for RNA extraction and hybridization on Affymetrix microarrays. We compared the expression profiles of parental U87 cells, U87 tumor sphere cells treated with vehicle (DMSO) and U87 tumor sphere cells treated with ITE.

2015-04-18 | E-GEOD-67986 | biostudies-arrayexpress

The UPR Transducer IRE1 Promotes Breast Cancer Malignancy by Degrading Tumor Suppressor microRNAs

Project description:To understand the mechanistic basis by which inositol-requiring enzyme 1 (IRE1) is involved in luminal breast cancer malignancy, we analyzed the transcriptomic signature that IRE1 regulates in breast cancer. We suppressed the activity of IRE1 RNase in luminal breast cancer SUM52 line by adenoviral-based over-expression of the IRE1 dominant-negative K599A or K907A, and then performed RNA-sequencing (RNA-seq) analysis with IRE1 dominant-negative and control SUM52 cells. Through the RNA-seq analysis, we identified 98 genes that were commonly upregulated (65 genes) or downregulated (33 genes) in K599A-expressing SUM52 (SUM52-K599A) or K907A-expressing SUM52 (SUM52-K907A) cells.

2021-01-15 | GSE156332 | GEO

Dual IRE1 RNase functions dictate glioblastoma development

Project description:The transcriptome dataset has been used to validate on a GBM patient cohort (n=117) the IRE1-dependent gene expression signature identified in U87 cell line.

2018-01-09 | E-MTAB-6326 | biostudies-arrayexpress

IRE1 modulates expression of a set of tumor suppressor miRNAs in luminal breast cancer cells.

Project description:To explore the regulatory mechanism by which inositol-requiring enzyme 1 (IRE1) regulates oncogenic factors, particularly RAB3B, in luminal breast cancer cells, we blocked IRE1 activity in breast cancer cell lines by using the IRE1 inhibitor 4μ8C or expressing IRE1 dominant-negative for miRNA microarray analysis. SUM52 and SUM225 lines with high-level IRE1 expression were treated with 4μ8C for 2 days. The IRE1 kinase dominant-negative mutant K599A or K907A was also used to suppress IRE1 kinase or RNase activity in SUM52 cells. The miRNA microarray analysis revealed a landscape change in miRNA expression profiling in IRE1-inhibited luminal breast cancer cells. Using a criterion of p < 0.05 in miRNA analysis, we identified 41 miRNAs in both SUM52 and SUM225 cells that were altered after inhibiting IRE1 activity. Additionally, we exogenously overexpressed wild-type IRE1 in human nontumorigenic mammary epithelial MCF10A cells and then performed miRNA array assays. When we combined miRNA data from both IRE1 inhibition models in breast cancer cells and exogenous overexpression of IRE1 in MCF10A cells (p<0.05), we identified 5 miRNAs (3607-3p, 374a-5p, 4764-3p, 516a-3p, and 6073) that were upregulated in IRE1-inhibited breast cancer cells and downregulated in MCF10A-IRE1 cells.

2021-01-15 | GSE156331 | GEO

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