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Gene profiling: U87 IRE1 dominant negative cells vs. U87ctrl cells in culture

ABSTRACT: Transcriptome analysis was performed from human U87 glioblastoma cell clones: U87 IRE1.NCK DN (U87dn, IRE1 dominant negative) and U87 control (U87ctrl, empty plasmid). Cells were grown in DMEM supplemented with 10% FBS and glutamine for 16 hours in culture prior mRNA isolation and analyses

ORGANISM(S): Homo sapiens

PROVIDER: GSE22385 | GEO | 2010/08/08

SECONDARY ACCESSION(S): PRJNA127735

REPOSITORIES: GEO

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Gene profiling: U87 IRE1 dominant negative cells vs. U87ctrl cells in culture

Project description:Transcriptome analysis was performed from human U87 glioblastoma cell clones: U87 IRE1.NCK DN (U87dn, IRE1 dominant negative) and U87 control (U87ctrl, empty plasmid). Cells were grown in DMEM supplemented with 10% FBS and glutamine for 16 hours in culture prior mRNA isolation and analyses U87dn cells expressing a dominant negative transgene of IRE1alpha were compared to U87ctrl cells transfected with the corresponding empty plamid to identify genes associated to tumor invasion and angiogenesis.

2010-08-08 | E-GEOD-22385 | biostudies-arrayexpress

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Project description:In this dataset, we included sequencing data of total and ribosome protected fragments obtained from PATU-8902 cell lines grown in DMEM (2.78mM glucose, 4mM glutamine, 1mM pyruvate) + 10%dialyzed FBS + 1% Pen/Strep with or without 400uM Ser and 400uM Gly for 24 hours.

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Transcription profiling of human U87 wild type, neo-resistant and PDGF-A dominant negative U87 gliobastoma cells to identify PDGF-dependent patterns of gene expression

Project description:Overall study: Identification of PDGF-dependent patterns of gene expression in U87 glioblastoma cells. RNA was obtained from triplicate dishes of 5 different groups of U87 cells, each (total 15) analyzed with one U95 microarray chip.,Three different comparisons were made:,1) Clone 3.1 (34580-34582) vs. clone 3.3 (34583-34585) vs. parent U87 (34592-34594).,Purpose: demonstrate that the gene expression profiles between these 3 cell lines are not different, so they could be pooled as a single untreated group.,2) Pooled control group (34580-34585, 34592-34594) vs. clone 8.1 (34586-34588).,Purpose: identify genes specifically controlled by autocrine PDGF activity.,3) Clone 8.1 (34586-34588),vs. clone 8.1 treated with PDGF (34589-34591),Purpose: Identify genes specifically induced by exogenous PDGF.

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