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Transcription profiling time series of HOXA5 induction in human Hs578T cell line

ABSTRACT: HOXA5 inducible cell line Hs578T was established and cultured as described previously (MCB 2004. 24:924-935). 2×106 cells were seeded onto a 10-cm cell culture dish at 24h before induction. Two batches of RNA from HOXA5-inducible cells (0 h, 6 h and 9 h) were purified for repeating the microarray hybridization experiments once using Affymatrix Chips.Total RNA was extracted using TRIzol reagent (Gibco BRL, Life Technologies, Grand Island, NY) and purified using the RNeasy Mini Kit (Qiagen, Valencia, CA).

ORGANISM(S): Homo sapiens

SUBMITTER: Hexin Chen 

PROVIDER: E-GEOD-2241 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Identification of transcriptional targets of HOXA5.

Chen Hexin H   Rubin Ethel E   Zhang Huiping H   Chung Seung S   Jie Charles C CC   Garrett Elizabeth E   Biswal Shyam S   Sukumar Saraswati S  

The Journal of biological chemistry 20050309 19

The homeobox gene HOXA5 encodes a transcription factor that has been shown to play important roles in embryogenesis, hematopoiesis, and tumorigenesis. In order to decipher downstream signaling pathways of HOXA5, we utilized oligonucleotide microarray analysis to identify genes that are differentially expressed in HOXA5-induced cells compared with uninduced cells. Comparative analysis of gene expression changes after 9 h of HOXA5 induction in Hs578T breast cancer cells identified 306 genes whose  ...[more]

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