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Control of mammary stem cell function by steroid hormone signalling.

ABSTRACT: This SuperSeries is composed of the following subset Series: GSE20401: Mammary epithelial cell subpopulations from control and ovariectomized mice GSE20402: Mouse mammary epithelial cell subpopulations from pregnant and virgin mice Refer to individual Series

ORGANISM(S): Mus musculus

SUBMITTER: Gordon Smyth

PROVIDER: E-GEOD-22446 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Control of mammary stem cell function by steroid hormone signalling.

Asselin-Labat Marie-Liesse ML Vaillant François F Sheridan Julie M JM Pal Bhupinder B Wu Di D Simpson Evan R ER Yasuda Hisataka H Smyth Gordon K GK Martin T John TJ Lindeman Geoffrey J GJ Visvader Jane E JE

Nature 20100411 7299

The ovarian hormones oestrogen and progesterone profoundly influence breast cancer risk, underpinning the benefit of endocrine therapies in the treatment of breast cancer. Modulation of their effects through ovarian ablation or chemoprevention strategies also significantly decreases breast cancer incidence. Conversely, there is an increased risk of breast cancer associated with pregnancy in the short term. The cellular mechanisms underlying these observations, however, are poorly defined. Here w ...[more]

PMID: 20383121

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Mammary epithelial cell subpopulations from control and ovariectomized mice

Project description:To study the effect of pregnancy on mouse mammary epithelial subpopulations, epthelial cells derived from control or ovariectomized adult mice were isolated using fluorescence-activated cell sorting. After elimination of haematopoietic and endothelial cells, two distinct epithelial subpopulations were sorted using antibodies against CD29 and CD24. Based on the immunohistochemical phenotype, and in vivo and in vitro functional assays, these subpopulations were identified as mammary stem cell enriched (CD29hiCD24+) and luminal (CD29loCD24+) respectively (ref: Shackleton et al, Nature 2006). Microarray profiling was used to compare gene expression profiles of the two subpopulations isolated from ovariectomized and control mice. For each of two biological replicates, 6 FVB/NJ mice were ovariectomized at 8 weeks of age. 4 weeks after ovariectomy (Ovx) mammary gland from control or Ovx animals were collected and digested to obtain a single cell suspension. CD45-CD31-TER119- cells were then sorted based on the expression of cell surface markers CD24 and CD29. Similarly for two pools of control mice. There were also 4 technical replicates, to make 12 BeadChips in total.

2010-04-10 | E-GEOD-20401 | biostudies-arrayexpress

Mouse mammary epithelial cell subpopulations from pregnant and virgin mice

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Expression data from sorted control and Pygo2-null mouse mammary stem/basal cells

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Mammary epithelial cell subpopulations from control and ovariectomized mice

2010-04-10 | GSE20401 | GEO

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2017-03-23 | E-MTAB-4145 | biostudies-arrayexpress

Mus musculus

Project description:Mammary epithelial cell subpopulations from control and ovariectomized mice

| PRJNA129827 | ENA

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2010-03-20 | E-GEOD-19446 | biostudies-arrayexpress

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