Unknown,Transcriptomics,Genomics,Proteomics

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Genome-wide transcription start site determination of Geobacter sulfurreducens under multiple growth conditions


ABSTRACT: Determine the transcription start sites at 1-bp resolution across the entire G. sulfurreducens genome in multiple growth conditions, including fresh water acetate-fumarate (FWAF) medium, nitrogen limitation and growth on electrode. Special capture of the 5' end of primary transcripts in G. sulfurreducens and sequencing with Illumina Genome Analyzer II.

ORGANISM(S): Geobacter sulfurreducens PCA

SUBMITTER: Bernhard Palsson 

PROVIDER: E-GEOD-22511 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Structural and operational complexity of the Geobacter sulfurreducens genome.

Qiu Yu Y   Cho Byung-Kwan BK   Park Young Seoub YS   Lovley Derek D   Palsson Bernhard Ø BØ   Zengler Karsten K  

Genome research 20100630 9


Prokaryotic genomes can be annotated based on their structural, operational, and functional properties. These annotations provide the pivotal scaffold for understanding cellular functions on a genome-scale, such as metabolism and transcriptional regulation. Here, we describe a systems approach to simultaneously determine the structural and operational annotation of the Geobacter sulfurreducens genome. Integration of proteomics, transcriptomics, RNA polymerase, and sigma factor-binding informatio  ...[more]

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