Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcriptome analysis of Geobacter sulfurreducens grown with different nitrogen sources

ABSTRACT: Investigation of comprehensive information about the expression level of RNA transcripts across the entire G.sulfurreducens genome with different nitrogen sources, including NH3, Gln, Asp and N2. A twelve chip study using total RNA recovered from four separate culture conditions of G.Sulfurreducens. G.Sulfurreducens PCA were harvested at mid-exponential phase. The high-density oligonucleotide tiling arrays used were consisted of 381,174 oligonucleotide probes spaced 20 bp apart (30-bp overlap between two probes) across the G.sulfurreducens genome (NimbleGen). Experiments were conducted as three bioliogical replicates (different cultures)

ORGANISM(S): Geobacter sulfurreducens PCA

SUBMITTER: Bernhard Palsson

PROVIDER: E-GEOD-17834 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Geobacter sulfurreducens grown in various conditions: gene expression and RpoN ChIP-chip

Project description:This SuperSeries is composed of the following subset Series: GSE17834: Transcriptome analysis of Geobacter sulfurreducens grown with different nitrogen sources GSE17837: ChIP-chip of Geobacter sulfurreducens PCA with antibody against RpoN under various conditions. Refer to individual Series

2010-06-28 | E-GEOD-17838 | biostudies-arrayexpress

Genome-wide transcription start site determination of Geobacter sulfurreducens under multiple growth conditions

Project description:Determine the transcription start sites at 1-bp resolution across the entire G. sulfurreducens genome in multiple growth conditions, including fresh water acetate-fumarate (FWAF) medium, nitrogen limitation and growth on electrode. Special capture of the 5' end of primary transcripts in G. sulfurreducens and sequencing with Illumina Genome Analyzer II.

2010-07-01 | E-GEOD-22511 | biostudies-arrayexpress

Analysis of Geobacter sulfurreducens under multiple growth conditions

Project description:This SuperSeries is composed of the following subset Series: GSE22497: Transcriptome analysis of Geobacter sulfurreducens under multiple growth conditions GSE22503: ChIP-chip of Geobacter sulfurreducens PCA with antibody against RNAP and RpoD under various conditions GSE22511: Genome-wide transcription start site determination of Geobacter sulfurreducens under multiple growth conditions Refer to individual Series

2010-06-30 | E-GEOD-22512 | biostudies-arrayexpress

ChIP-chip of Geobacter sulfurreducens PCA with antibody against RpoN under various conditions.

Project description:We applied a ChIP-chip approach to elucidate the σN regulon experimentally under different growth conditions. This technique localizes DNA fragments within σN complexes enriched by chromatin immunoprecipitation using high-density oligonucleotide tilling arrays. A nine ChIP-chip study using immunoprecipitated DNA (IP-DNA) from three separate culture conditions. The high-density oligonucleotide tiling arrays used were consisted of 381,174 oligonucleotide probes spaced 20 bp apart (30-bp overlap between two probes) across the G.sulfurreducens genome (NimbleGen). Experiments were conducted as three bioliogical replicates (different cultures)

2010-06-30 | E-GEOD-17837 | biostudies-arrayexpress

ChIP-chip of Geobacter sulfurreducens PCA with antibody against RNAP and RpoD under various conditions

Project description:We applied a ChIP-chip approach to elucidate the binding profiles of RNAP and RpoD experimentally under different growth conditions. This technique localizes DNA fragments within DNA-protein complexes enriched by chromatin immunoprecipitation using high-density oligonucleotide tilling arrays. A 21 ChIP-chip study using immunoprecipitated DNA (IP-DNA) from three culture conditions for RNAP and four culture conditions for RpoD. The high-density oligonucleotide tiling arrays used consisted of 381,174 oligonucleotide probes spaced 20 bp apart (30-bp overlap between two probes) across the G. sulfurreducens genome (NimbleGen). Experiments were conducted as three bioliogical replicates (different cultures).

2010-07-01 | E-GEOD-22503 | biostudies-arrayexpress

ChIP-chip of Geobacter sulfurreducens PCA with antibody against various sigma factors under various conditions

Project description:We applied a ChIP-chip approach to elucidate the binding profiles of sigma factors (RpH, RpoN and RpoS) experimentally under different growth conditions. This technique localizes DNA fragments within DNA-protein complexes enriched by chromatin immunoprecipitation using high-density oligonucleotide tilling arrays. A 10 ChIP-chip study using immunoprecipitated DNA (IP-DNA) from three culture condtions against three alternative sigma factors . The high-density oligonucleotide tiling arrays used were consisted of 381,174 oligonucleotide probes spaced 20 bp apart (30-bp overlap between two probes) across the G.sulfurreducens genome (NimbleGen). Experiments were conducted as three bioliogical replicates (different cultures).

2013-05-01 | E-GEOD-37778 | biostudies-arrayexpress

Transcriptome analysis of Geobacter sulfurreducens under multiple growth conditions

Project description:Investigation of comprehensive information about the transcripts (boundary, level, etc.) across the entire G. sulfurreducens genome in mulitple growth conditions, including in biofilm and on electrode. A five array study using total RNA recovered from two separate culture conditions of G. sulfurreducens. G. sulfurreducens were harvested one week after growth on electrode or to form biofilm. The high-density oligonucleotide tiling arrays used consisted of 381,174 oligonucleotide probes spaced 20 bp apart (30-bp overlap between two probes) across the G. sulfurreducens genome (NimbleGen). Experiments were conducted as two (electrode) or three (biofilm) biological replicates (different cultures).

2010-07-01 | E-GEOD-22497 | biostudies-arrayexpress

Proteome reference map of Haloarcula hispanica and comparative proteomic and transcriptomic analysis of polyhydroxyalkanoate biosynthesis under genetic and environmental perturbations

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2013-01-22 | E-GEOD-42800 | biostudies-arrayexpress

Genes differentially expressed Haloarcula hispanica strains: PHB (M-NM-^TphaEC mutant) vs 960 (wild type)

Project description:Transcriptional profiling of Haloarcula hispanica comparing PHB with 960. PHB represents the PHA synthase genes mutant (i.e., M-NM-^TphaEC) which is deficient in PHBV accumulation when grown in MG medium. 960 represents the wild-type strain which can produce PHBV when grown in MG medium. Goal was to explore the PHBV biosynthesis and its impact on central metabolism in H. hispanica. Total RNA from PHB and 960 were used to generate target cDNA, and then hybridized to 8*15K Haloarcula hispanica genome array genechips, representing about 3800 genes.

2013-01-22 | E-GEOD-42799 | biostudies-arrayexpress

Minimal metabolic pathway structure is consistent with associated macromolecular interactions.

Project description:We sequenced mRNA of three dual perturbation experiments of E. coli K12 MG1655 changing both genetic and environmental conditions. Each dual perturbation experiment consists of four RNA-seq samples (WT, WT+nutrient supplementation, transcription factor KO, transcription factor KO+nutrient supplementation). The three conditions are: 1) adenine supplementation/nac KO, 2) L-tryptophan supplementation/cra KO, 3) anaerobic/mntR KO. Determination of whether a transcription factor plays a regulatory role in a particular environmental shift by examining differential expression of mRNA levels.

2014-06-30 | E-GEOD-48324 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data