Unknown,Transcriptomics,Genomics,Proteomics

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Control of Embryonic Stem Cell State by Mediator and Cohesin (Agilent gene expression data)


ABSTRACT: The key transcription factors that control the embryonic stem cell gene expression program have been identified, but how they function to implement this program is not well understood. While screening for genes essential for maintenance of ES cell state, we identified many components of the Mediator and Cohesin complexes. Mediator and Cohesin were found to physically and functionally connect the enhancers and core promoters of active genes. An ES cell Mediator complex was found to copurify with Cohesin and its loading factor Nipbl, and normal levels of these proteins were essential for expression of the genes they occupy and for maintenance of ES cell state. See associated publication.

ORGANISM(S): Mus musculus

SUBMITTER: Richard Young 

PROVIDER: E-GEOD-22556 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications


Transcription factors control cell-specific gene expression programs through interactions with diverse coactivators and the transcription apparatus. Gene activation may involve DNA loop formation between enhancer-bound transcription factors and the transcription apparatus at the core promoter, but this process is not well understood. Here we report that mediator and cohesin physically and functionally connect the enhancers and core promoters of active genes in murine embryonic stem cells. Mediat  ...[more]

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