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Human BJ fibroblasts containing control vs. BRD7 shRNA

ABSTRACT: Transcriptional profiling of human BJ fibroblasts comparing control FF shRNA expressing cells vs. BRD7 shRNA expressing cells under two conditions, either untreated or treated with 8uM nutln-3a for 8 hours. This experiment was done using two independent shRNAs targeting BRD7. Nutlin-3a was used to stabilize p53 and induce its transcriptional activity. Two-condition experiment, FF shRNA cells vs. BRD7 shRNAs cells in two experimental conditions, either untreated or treated with nutlin-3a.

ORGANISM(S): Homo sapiens

SUBMITTER: Anna Burrows

PROVIDER: E-GEOD-22607 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Polybromo-associated BRG1-associated factor components BRD7 and BAF180 are critical regulators of p53 required for induction of replicative senescence.

Burrows Anna E AE Smogorzewska Agata A Elledge Stephen J SJ

Proceedings of the National Academy of Sciences of the United States of America 20100726 32

A variety of tumor-suppressor mechanisms exist to promote genome integrity and organismal survival. One such mechanism is cellular senescence. In response to replicative aging, DNA damage, and oncogenic stimuli, the p53 and Rb pathways are activated to prevent the proliferation of damaged cells by inducing senescence or apoptosis. We have performed a loss-of-function genetic screen in primary human cells to identify components of the senescence machinery. Here we describe BRD7 and BAF180 as uniq ...[more]

PMID: 20660729

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