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Global analysis of p53-regulated transcription reveals its direct targets and unexpected regulatory mechanisms (GRO-Seq)

ABSTRACT: GRO-seq of HCT116 cells treated with Nutlin to activate p53 HCT116 cells were treated with Nutlin or DMSO for 1 hour

ORGANISM(S): Homo sapiens

SUBMITTER: Mary Allen

PROVIDER: E-GEOD-53964 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Global analysis of p53-regulated transcription identifies its direct targets and unexpected regulatory mechanisms.

Allen Mary Ann MA Andrysik Zdenek Z Dengler Veronica L VL Mellert Hestia S HS Guarnieri Anna A Freeman Justin A JA Sullivan Kelly D KD Galbraith Matthew D MD Luo Xin X Kraus W Lee WL Dowell Robin D RD Espinosa Joaquin M JM

eLife 20140527

The p53 transcription factor is a potent suppressor of tumor growth. We report here an analysis of its direct transcriptional program using Global Run-On sequencing (GRO-seq). Shortly after MDM2 inhibition by Nutlin-3, low levels of p53 rapidly activate ∼200 genes, most of them not previously established as direct targets. This immediate response involves all canonical p53 effector pathways, including apoptosis. Comparative global analysis of RNA synthesis vs steady state levels revealed that mi ...[more]

PMID: 24867637

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