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Myc-regulated gene expression in human fibroblasts

ABSTRACT: Myc regulates many gene expression during cell growth, development, or other many cellular events. C-Myc is a well known molecule which expression increase in many cancers. Overexpression of c-Myc causes cell transformation, apoptosis, etc. There are several Myc family genes in human, mouse, or other species. The function of N-Myc was well characterized and was similar to that of c-Myc. However, the function of L-Myc is not clearly examined. To examine the function of L-Myc, we perfomed microarray analyses using L-Myc overexpressed human fibroblasts. Adult human fibroblasts were transduced with Myc and their mutants and were used for microarray analyses.

ORGANISM(S): Homo sapiens

SUBMITTER: Shinya Yamanaka

PROVIDER: E-GEOD-22654 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Promotion of direct reprogramming by transformation-deficient Myc.

Nakagawa Masato M Takizawa Nanako N Narita Megumi M Ichisaka Tomoko T Yamanaka Shinya S

Proceedings of the National Academy of Sciences of the United States of America 20100726 32

Induced pluripotent stem cells (iPSCs) are generated from mouse and human fibroblasts by the introduction of three transcription factors: Oct3/4, Sox2, and Klf4. The proto-oncogene product c-Myc markedly promotes iPSC generation, but also increases tumor formation in iPSC-derived chimeric mice. We report that the promotion of iPSC generation by Myc is independent of its transformation property. We found that another Myc family member, L-Myc, as well as c-Myc mutants (W136E and dN2), all of which ...[more]

PMID: 20660764

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