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Coupling p53 binding and nucleosome occupancy measurements at p53 binding sites

ABSTRACT: We were interested to explain why p53 binds some high affinity sites in contrast to other high affinity sites that are not bound by p53. p53 binding was measured using p53 ChIP-CHIP and in parallel nucleosome occupancy was measured on these same sites Comparison between p53 binding and nucleosome occupancy at p53 predicted binding sites ChIP-CHIP of p53 from MCF7EcoR under Basal conditions and MCF7EcoR treated with NCS (Activated) and Mononucleosomal extraction from MCF7sip53, MCF7EcoR under Basal conditions and MCF7EcoR treated with NCS (Activated) Expression analysis of MCF7sip53 and MCF7EcoR treated with NCS (Activated)

ORGANISM(S): Homo sapiens

SUBMITTER: Efrat Lidor Nili

PROVIDER: E-GEOD-22783 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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p53 binds preferentially to genomic regions with high DNA-encoded nucleosome occupancy.

Lidor Nili Efrat E Field Yair Y Lubling Yaniv Y Widom Jonathan J Oren Moshe M Segal Eran E

Genome research 20100817 10

The human transcription factor TP53 is a pivotal roadblock against cancer. A key unresolved question is how the p53 protein selects its genomic binding sites in vivo out of a large pool of potential consensus sites. We hypothesized that chromatin may play a significant role in this site-selection process. To test this, we used a custom DNA microarray to measure p53 binding at approximately 2000 sites predicted to possess high-sequence specificity, and identified both strongly bound and weakly bo ...[more]

PMID: 20716666

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