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Cell type-specific chromatin profiling of Drosophila neurons [ChIP-Seq]

ABSTRACT: Chromatin profiling of nuclei isolated from genetically defined neuronal subpopulations of the adult Drosophila brain. Cell type-specific histone modification maps were generated from nuclei isolated from all neurons (R57C10-GAL4), Kenyon cells (OK107-GAL4), and octopaminergic (Tdc2-GAL4) neurons using a method similar to INTACT (Deal and Henikoff, 2010; Steinner et al., 2012). Three histone modifications were profiled: H3K4me3, H3K27ac, and H3K27me3. Sequencing was performed with an Illumina HiSeq 2000.

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Fred Davis

PROVIDER: E-GEOD-37032 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Cell type-specific genomics of Drosophila neurons.

Henry Gilbert L GL Davis Fred P FP Picard Serge S Eddy Sean R SR

Nucleic acids research 20120801 19

Many tools are available to analyse genomes but are often challenging to use in a cell type-specific context. We have developed a method similar to the isolation of nuclei tagged in a specific cell type (INTACT) technique [Deal,R.B. and Henikoff,S. (2010) A simple method for gene expression and chromatin profiling of individual cell types within a tissue. Dev. Cell, 18, 1030-1040; Steiner,F.A., Talbert,P.B., Kasinathan,S., Deal,R.B. and Henikoff,S. (2012) Cell-type-specific nuclei purification f ...[more]

PMID: 22855560

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