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Expression data from roots and first two leaves of tomato seedlings growing on regular MS medium or MS medium supplemented with multi-wall carbon nanotubes (0, 50, 100, 200 ug/ml) or activated carbon (50 mg/ml)

ABSTRACT: To investigate effects of carbon nanotubes (CNTs) om total expression profile of tomato plants we introduced CNTs in MS agar medium in concentrationd 0, 50, 100, 200 ug/ml Objectives for this study included the identification of genes that were up or down-regulated at the transcriptional level in tomato seedlings in response to carnom nanotubes (CNTs). 10 day old root tips and first two leaves of wild type tomato seedlings (cv. Micro-Tom) growing on regular MS agar medium or MS medium supplemented with carbon nanotubes (50, 100, 200 ug/ml) or MS medium supplemented with activated carbon (50 ug/ml) were selected for RNA extraction and hybridization on Affymetrix microarrays.

ORGANISM(S): Solanum lycopersicum

SUBMITTER: Mariya Khodakovskaya

PROVIDER: E-GEOD-22803 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Expression data from roots and first two leaves of tomato seedlings growing on regular MS medium or MS medium supplemented with multi-wall carbon nanotubes (0, 50, 100, 200 ug/ml) or activated carbon (50 mg/ml)

Project description:To investigate effects of carbon nanotubes (CNTs) om total expression profile of tomato plants we introduced CNTs in MS agar medium in concentrationd 0, 50, 100, 200 ug/ml Objectives for this study included the identification of genes that were up or down-regulated at the transcriptional level in tomato seedlings in response to carnom nanotubes (CNTs).

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Expression analysis of seeds and seedlings of tomato growing on regular MS medium or MS medium supplemented with Helical multi-walled carbon nanotubes (Helical MWCNTs) in concentration 25 ug/ml

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In vivo pulmonary genotoxicity of multiwalled-carbon nanotubes

Project description:Carbon nanotubes (CNTs) are newly developed nanomaterials with unique chemical and physical properties. Exposure to airborne CNTs in occupational settings or via consumer products is expected to increase significantly within the next decade due to the vigorous synthesis and applications of these materials in numerous consumer and industrial activities. Previous studies have shown that multiwalled CNT (MWCNT) induce pulmonary inflammation and pulmonary fibrosis. In the present study, we investigated genotoxic potential of MWCNTs. Female MutaMouse were exposed to 42.7 ug/mouse or 128 ug/mouse doses of MWCNTs Mitsui XNRi-7 or NM 401 once a week for four consecutive weeks. Doses were administered via intratracheal instillation. Lung tissues were collected 56 days post-exposure. Bronchoalveolar lavage(BAL) fluid cellularity, BAL and lung tissue DNA damage (COMET assay), lacz mutation frequency and global gene expression changes in lung tissue were determined.

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Solanum lycopersicum

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Profiling of gene expression in lung tissue in C57Bl/6 and DBA/2 mouse strains following exposure to carbon nanotubes

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