Unknown,Transcriptomics,Genomics,Proteomics

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Sequencing of the non-ribosomal transcriptome allows the simultaneous identification of protein-coding and non-protein-coding RNAs


ABSTRACT: Deep Sequencing of protein-coding and non-protein-coding RNAs from mouse differentiated embryonic stem cells and 14.5dpc mouse fetal head Analysis of Ribominus RNA from mouse differentiated embryonic stem cells and 14.5dpc mouse fetal head There are no processed data files for GSM566806-GSM566811. There are no fastq raw data files for GSM566812 and GSM566813 since these samples are the combined reads from all sequence lanes.

ORGANISM(S): Mus musculus

SUBMITTER: Ru Huang 

PROVIDER: E-GEOD-22959 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

An RNA-Seq strategy to detect the complete coding and non-coding transcriptome including full-length imprinted macro ncRNAs.

Huang Ru R   Jaritz Markus M   Guenzl Philipp P   Vlatkovic Irena I   Sommer Andreas A   Tamir Ido M IM   Marks Hendrik H   Klampfl Thorsten T   Kralovics Robert R   Stunnenberg Hendrik G HG   Barlow Denise P DP   Pauler Florian M FM  

PloS one 20111110 11


Imprinted macro non-protein-coding (nc) RNAs are cis-repressor transcripts that silence multiple genes in at least three imprinted gene clusters in the mouse genome. Similar macro or long ncRNAs are abundant in the mammalian genome. Here we present the full coding and non-coding transcriptome of two mouse tissues: differentiated ES cells and fetal head using an optimized RNA-Seq strategy. The data produced is highly reproducible in different sequencing locations and is able to detect the full le  ...[more]

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