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Gene expression profiling of HUVEC after stimulation with an inflammatory mediator cocktail

ABSTRACT: To understand the transcripts regulated by inflammation and how this regulation may differ between different individuals, the transcriptome of HUVECs stimulated with inflammatory mediators was examined in 9 different individuals. In the study present here, HUVEC isolates from 9 different individuals were cultured until passage 4 in fully supplemented growth conditions. Passage 4 HUVEC isolates were then treated with a cocktail of inflammatory mediators (10ng/ml TNF-α, Il-1β, Il-8) for a period of 24 hours prior to RNA extraction. RNA was extracted and hybridised onto CodeLink microarrays. The expression profiles in the inflammatory mediator treated condition were examined along side a superset of the same biological replicates that were untreated (also submitted), to facilitate assessment of the resting transcriptome.

ORGANISM(S): Homo sapiens

SUBMITTER: Muna Affara

PROVIDER: E-GEOD-23069 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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