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MicroRNA expression in human tonsillar B cell populations

ABSTRACT: small RNA profiles of 6 human tonsillar B cell populatios (naive B cells, pre-germinal center B cells, centrocytes, centroblasts, memory B cells, and plasma cells) were determined by deep sequencing. These samples were compared to mouse developing lymphocytes, various hematopoietic cell lineages, and tissues. small RNA expression profiles of 6 well defined B cell populations isolated from human tonsils.

ORGANISM(S): Homo sapiens

SUBMITTER: Wolfgang Resch

PROVIDER: E-GEOD-23090 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Regulation of microRNA expression and abundance during lymphopoiesis.

Kuchen Stefan S Resch Wolfgang W Yamane Arito A Kuo Nan N Li Zhiyu Z Chakraborty Tirtha T Wei Lai L Laurence Arian A Yasuda Tomoharu T Peng Siying S Hu-Li Jane J Lu Kristina K Dubois Wendy W Kitamura Yoshiaki Y Charles Nicolas N Sun Hong-wei HW Muljo Stefan S Schwartzberg Pamela L PL Paul William E WE O'Shea John J Rajewsky Klaus K Casellas Rafael R

Immunity 20100603 6

Although the cellular concentration of miRNAs is critical to their function, how miRNA expression and abundance are regulated during ontogeny is unclear. We applied miRNA-, mRNA-, and ChIP-Seq to characterize the microRNome during lymphopoiesis within the context of the transcriptome and epigenome. We show that lymphocyte-specific miRNAs are either tightly controlled by polycomb group-mediated H3K27me3 or maintained in a semi-activated epigenetic state prior to full expression. Because of miRNA ...[more]

PMID: 20605486

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