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Genome-wide analysis of time-dependent gene expression in MCF-10HER-2 cells treated with the EGFR-specific inhibitor gefitinib for 45 hours

ABSTRACT: Results of blocking activated EGFR/HER-2 heterodimer signaling in MCF-10HER-2 cells by treatment with gefitinib and measuring gene expression as a function of time provides information as to what genes are regulated by EGFR/HER-2 signaling in these breast epithelial cells that are transduced to overexpress the HER-2 oncogene and express transformed phenotypes. MCF-10HER-2 cells were treated with the EGFR-specific small molecule kinase inhibitor gefitinib for 45 hours. Total RNA was collected every 3 hours from parallel cultures throughout the 45 hour duration of treatment and genome-wide analysis of expression was performed on RNA from each time point (total 16 time points, starting at 0 hours treatment).

ORGANISM(S): Homo sapiens

SUBMITTER: Aliccia Bollig-Fischer

PROVIDER: E-GEOD-23136 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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HER-2 signaling, acquisition of growth factor independence, and regulation of biological networks associated with cell transformation

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Homo sapiens

Project description:Genome-wide analysis of time-dependent gene expression in MCF-10A cells treated with the EGFR-specific inhibitor gefitinib for 45 hours

| PRJNA133237 | ENA

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