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Expression analysis of sporulating Bacillus subtilis strain 168 deleted for prpE gene

ABSTRACT: Investigation of whole genome gene expression level changes in sporulating Bacillus subtilis 168 delta-prpE mutant, compared to the wild-type strain. The mutation engineered into this strain results in impaired germination of spores. A six chip study using total RNA extracted from three separate wild-type cultures of sporulating Bacillus subtilis 168 and three separate cultures of sporulating mutant strain, Bacillus subtilis 168 delta-prpE, in which prpE (yjbP BSU11630) gene coding for a protein phosphatase is deleted entirely. Each chip consists of four fields able to measure the expression level of 4,104 genes from Bacillus subtilis subsp. subtilis strain 168 NC_000964 with eight 60-mer probe pairs (PM/MM) per gene, with two-fold technical redundancy.

ORGANISM(S): Bacillus subtilis subsp. subtilis str. 168

SUBMITTER: Adam Iwanicki

PROVIDER: E-GEOD-44125 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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A genome-wide transcriptional profiling of sporulating Bacillus subtilis strain lacking PrpE protein phosphatase.

Iwanicki Adam A Hinc Krzysztof K Ronowicz Anna A Piotrowski Arkadiusz A Wołoszyk Aleksandra A Obuchowski Michał M

Molecular genetics and genomics : MGG 20130704 10

The sporulation process is a complex genetic developmental program leading to profound changes in global gene expression profile. In this work, we have applied genome-wide microarray approach for transcriptional profiling of Bacillus subtilis strain lacking a gene coding for PrpE protein phosphatase. This protein was previously shown to be involved in the regulation of germination of B. subtilis spores. Moreover, the deletion of prpE gene resulted in changing the resistance properties of spores. ...[more]

PMID: 23824080

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Expression analysis of sporulating Bacillus subtilis strain 168 deleted for prpE gene

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