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Expression data in single and double knockouts of AtNHX5 and AtNHX6 in Arabidopsis

ABSTRACT: We generated single and double knockouts of AtNHX5 and AtNHX6 in order to invesigate possible functions in Arabidopsis. nhx5/nhx6 exhibited severe growth retardation related to cell size and proliferation, as well as endosomal trafficking perutrbations. The results implicate endosomal NHX antiporters in novel cellular functions. In order to investigate further the possible functions of AtNHX5 and AtNHX6, we compared the transcrptional profiles of single and double AtNHX5 and AtNHX6 knockouts. We looked for changes in gene expression might help us to elucidate the molecular events associated with the apparent requirement of AtNHX5 and AtNHX6 for normal growth and development. Rosette leaves from 4 weeks old plants grown on soil under normal conditons and 8h L / 16 D were collected for RNA extraction and hybridization on Affymetrix microarray chips.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: elias bassil 

PROVIDER: E-GEOD-23210 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The Arabidopsis intracellular Na+/H+ antiporters NHX5 and NHX6 are endosome associated and necessary for plant growth and development.

Bassil Elias E   Ohto Masa-aki MA   Esumi Tomoya T   Tajima Hiromi H   Zhu Zhu Z   Cagnac Olivier O   Belmonte Mark M   Peleg Zvi Z   Yamaguchi Toshio T   Blumwald Eduardo E  

The Plant cell 20110128 1

Intracellular Na(+)/H(+) antiporters (NHXs) play important roles in cellular pH and Na(+) and K(+) homeostasis in all eukaryotes. Based on sequence similarity, the six intracellular Arabidopsis thaliana members are divided into two groups. Unlike the vacuolar NHX1-4, NHX5 and NHX6 are believed to be endosomal; however, little data exist to support either their function or localization. Using reverse genetics, we show that whereas single knockouts nhx5 or nhx6 did not differ from the wild type, t  ...[more]

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