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Unknown,Transcriptomics,Genomics,Proteomics

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Mapping of H3K27me3 enrichment in Drosophila larval brain tissue using ChIP-chip

ABSTRACT: We made Polycomb (PC) and histone H3 lysine 27 trimethylation (H3K27me3) chromatin binding maps in central brain tissue from 3rd instar larvae, allowing us to make a direct comparison to our 4C data (http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE23166). Our results demonstrate that our PC and H3K27me3 maps are highly similar, and fit with previously identified hallmarks of PcG-bound chromatin, namely: PC and H3K27me3 co-occur in the genome as large contiguous domains that largely repress transcription of the underlying genes, which encode important regulators of development. Keywords: Genome binding/occupancy profiling by genome tiling array ChIP-chip experiments for H3K27me3 were performed in Drosophila larval brain tissue. Samples were hybridized to 380k NimbleGen arrays with 300 bp probe spacing.

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Maarten van Lohuizen

PROVIDER: E-GEOD-23299 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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