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Aldosterone-Regulated Expression Data in Mouse Aorta

ABSTRACT: The steroid hormone aldosterone plays a role in vascular function and disease. Aldosterone activates the mineralocorticoid receptor (MR), a ligand-activated transcription factor. MR have been found to be expressed in vascular cells and vessels. We used microarrays to identify the global programme of gene expression changes in mouse aortas treated with vehicle (DMSO) or aldosterone. Wild type C57Bl6 male mice were treated with spironolactone (20 mg/kg/day, an MR antagonist) for 5 days by Sub-cutaneous pellet to suppress basal MR-mediated gene expression. Aortas were then harvested and treated ex vivo in organ culture with vehicle or 100 nM aldosterone for 2, 4, or 8 hours. Three aortas were pooled for each timepoint and treatment, and three pooled biological replicates were performed.

ORGANISM(S): Mus musculus

SUBMITTER: Lakshmanan Iyer 

PROVIDER: E-GEOD-23566 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Placental growth factor mediates aldosterone-dependent vascular injury in mice.

Jaffe Iris Z IZ   Newfell Brenna G BG   Aronovitz Mark M   Mohammad Najwa N NN   McGraw Adam P AP   Perreault Roger E RE   Carmeliet Peter P   Ehsan Afshin A   Mendelsohn Michael E ME  

The Journal of clinical investigation 20101101 11

In clinical trials, aldosterone antagonists reduce cardiovascular ischemia and mortality by unknown mechanisms. Aldosterone is a steroid hormone that signals through renal mineralocorticoid receptors (MRs) to regulate blood pressure. MRs are expressed and regulate gene transcription in human vascular cells, suggesting that aldosterone might have direct vascular effects. Using gene expression profiling, we identify the pro-proliferative VEGF family member placental growth factor (PGF) as an aldos  ...[more]

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