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Cistrome of the Aldosterone-activated Mineralocorticoid Receptor in Human Renal Cells

ABSTRACT: Aldosterone, the main mineralocorticoid hormone in Humans, has a major role in maintaining the hemo-electrolytique homeostasis, by acting in the distal part of nephron. This steroid hormone mainly acts through its binding to a ligand-induced transcription factor, the mineralocorticoid receptor (MR). MR binds to specific genomic sequences, where it recruits transcriptional coregulators, to activate or repress target gene transcription. The aim of this work was to access the whole aldosterone-dependand MR target genes, by comparing sequenced data from aldosterone or vehicle-treated samples. Anti-MR ChIP-seq in Human Kidney GFP-hMR cells treated with vehicle or Aldosterone.

ORGANISM(S): Homo sapiens

SUBMITTER: Florian Le Billan

PROVIDER: E-GEOD-69777 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Cistrome of the aldosterone-activated mineralocorticoid receptor in human renal cells.

Le Billan Florian F Khan Junaid A JA Lamribet Khadija K Viengchareun Say S Bouligand Jérôme J Fagart Jérôme J Lombès Marc M

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 20150608 9

Aldosterone exerts its effects mainly by activating the mineralocorticoid receptor (MR), a transcription factor that regulates gene expression through complex and dynamic interactions with coregulators and transcriptional machinery, leading to fine-tuned control of vectorial ionic transport in the distal nephron. To identify genome-wide aldosterone-regulated MR targets in human renal cells, we set up a chromatin immunoprecipitation (ChIP) assay by using a specific anti-MR antibody in a different ...[more]

PMID: 26054365

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