Unknown,Transcriptomics,Genomics,Proteomics

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Gene expression data from FLCN-restored UOK257-2 renal cancer cells

ABSTRACT: UOK257 cell line was derived from a BHD patient. It harbors a germline mutation in FLCN (c.1285dupC) and LOH. UOK257-2 cells were generated from UOK257 cells by introducing wildtype FLCN using retrovirus. FLCN inactivation induces TFE3 transcriptional activity by increasing its nuclear localization. Thus expression microarray was used to identify the genes regulated by FLCN and TFE3. UOK257-2 cells expressing wildtype FLCN were transfected with 1) scrambled, 2) FLCN, 3) TFE3 and 4)FLCN/TFE3 siRNAs with Lipofectamine 2000 transfection reagent. Cells were harvested 3 days after transfection and RNAs were isolated using Trizol reagent and RNeasy Mini Kit. The genes induced by FLCN siRNA and reduced by TFE3 siRNA were chosen as a candidate genes regulated by FLCN and TFE3.

ORGANISM(S): Homo sapiens

SUBMITTER: William Linehan 

PROVIDER: E-GEOD-23614 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

The UOK 257 cell line: a novel model for studies of the human Birt-Hogg-Dubé gene pathway.

Yang Youfeng Y   Padilla-Nash Hesed M HM   Vira Manish A MA   Abu-Asab Mones S MS   Val Daniel D   Worrell Robert R   Tsokos Maria M   Merino Maria J MJ   Pavlovich Christian P CP   Ried Thomas T   Linehan W Marston WM   Vocke Cathy D CD  

Cancer genetics and cytogenetics 20080101 2

The establishment, characterization, and tumorigenicity of a new epithelial cell line (UOK 257) derived from human renal carcinoma of an individual with Birt-Hogg-Dubé (BHD) syndrome are reported. Unlike other established renal tumor cell lines from sporadic renal cell carcinoma, this is the first established renal tumor cell line of BHD, an inheritable neoplastic syndrome. The isolated tumor cells display loss of contact inhibition in vitro, and produce subcutaneous tumors in mouse xenografts.  ...[more]

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