Unknown,Transcriptomics,Genomics,Proteomics

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Tfe3-bound regions in naive pluripotent mouse embryonic stem (ES) cells


ABSTRACT: The bHLH transcription factor Tfe3 is a powerful regulator of pluripotency and we report a genome-wide analysis of Tfe3 occupancy in mouse ES cells. Nuclear localization of Tfe3 is inhibited by a protein complex containing the tumor-suppressor Folliculin (Flcn) and we also determine Tfe3 binding sites in ES cells expressing an shRNA targeting Flcn. Specificity is controlled for by using unspecific IgGs and ES cells expressing an shRNA targeting Tfe3. ChIP-Seq profiling of Tfe3 in ES cells

ORGANISM(S): Mus musculus

SUBMITTER: Joerg Betschinger 

PROVIDER: E-GEOD-39815 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Exit from pluripotency is gated by intracellular redistribution of the bHLH transcription factor Tfe3.

Betschinger Joerg J   Nichols Jennifer J   Dietmann Sabine S   Corrin Philip D PD   Paddison Patrick J PJ   Smith Austin A  

Cell 20130401 2


Factors that sustain self-renewal of mouse embryonic stem cells (ESCs) are well described. In contrast, the machinery regulating exit from pluripotency is ill defined. In a large-scale small interfering RNA (siRNA) screen, we found that knockdown of the tumor suppressors Folliculin (Flcn) and Tsc2 prevent ESC commitment. Tsc2 lies upstream of mammalian target of rapamycin (mTOR), whereas Flcn acts downstream and in parallel. Flcn with its interaction partners Fnip1 and Fnip2 drives differentiati  ...[more]

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