Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Genome wide expression analysis of psaA and psbA tobacco mutant plants.

ABSTRACT: Transcriptome analysis in tobacco mutant plants using tomato Genechip Genome array Tobacco (Nicotiana tabacum cv. Petit Havanna) psaA and psbA deletion mutants were constructed through a targeted deletion of 767 and 1152 nucleotides of coding regions, respectively with two gene cassettes: psbAproR:uidA:psbterR and rrnR:aadA:rbcLterR coding for GUS reporter and spectinomycin selectable marker genes, respectively. Standard established procedures were followed for chloroplast transformation to generate the psaA and psbA deletion mutants based on the homologous recombination. Gene expression profiles in psaA and psbA tobacco mutant plants were analyzed using tomato Genechip Genome array to study the global changes in the expression of genome. Total RNA was isolated from psaA and psbA tobacco mutant plants along with the wild type plants. Biotin labeled cRNA was hybridized on tomato GeneChip Genome Array following the Affymetrix protocols. Two independent biological replicates were maintained.

ORGANISM(S): Nicotiana tabacum

SUBMITTER: K.v. latha

PROVIDER: E-GEOD-23626 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Genome-wide transcriptome and proteome analyses of tobacco psaA and psbA deletion mutants.

Leelavathi Sadhu S Bhardwaj Amit A Kumar Saravanan S Dass Abhishek A Pathak Ranjana R Pandey Shiv S SS Tripathy Baishnab C BC Padmalatha K V KV Dhandapani Gurusamy G Kanakachari Mogilicherla M Solanke Amolkumar Uddhaorao AU Kumar Polumetla Ananda PA Cella Rino R Siva Reddy V V

Plant molecular biology 20110214 3-5

Photosynthesis in higher land plants is a complex process involving several proteins encoded by both nuclear and chloroplast genomes that require a highly coordinated gene expression. Significant changes in plastid differentiation and biochemical processes are associated with the deletion of chloroplast genes. In this study we report the genome-wide responses caused by the deletion of tobacco psaA and psbA genes coding core components of photosystem I (PSI) and photosystem II (PSII), respectivel ...[more]

PMID: 21327516

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Dataset Information

Genome wide expression analysis of psaA and psbA tobacco mutant plants.

Publications

Genome-wide transcriptome and proteome analyses of tobacco psaA and psbA deletion mutants.

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