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Raw data sets for Identification of OsHSP70 as a key regulator of MSP1-induced signaling in rice

ABSTRACT: Magnaporthe oryzae snodprot1 homolog (MSP1), a recently identified PAMP, is known to trigger defense responses in rice. However, the receptor(s) and downstream interacting components of MSP1 in rice have not been identified so far. Using multiple immunoprecipitation-mass spectrometry (IP-MS) analyses in transgenic rice expressing MSP1-YFP, we identified several heat shock proteins (HSPs) as MSP1 interacting partners. Heat stress treatment suppressed MSP1-induced cell death in tobacco plants transiently expressing MSP1. Subsequent IP-MS analysis on these tobacco plants, exposed to heat stress, resulted in the identification of a HSP having a highly conserved sequence to the OsHSP70 of rice. Co-expression of MSP1 with OsHSP70 failed to induce cell death in tobacco, confirming the involvement of the OsHSP70 in the MSP1 immune response. Rice protoplasts, co-expressing MSP1 and OsHSP70, showed a direct interaction between these two proteins in planta. Taken together, the results reported here highlight the pivotal role of OsHSP70 in MSP1-induced signaling and enhance our understanding of rice-M. oryzae interaction.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Oryza Sativa (rice)

TISSUE(S): Leaf, Shoot

SUBMITTER: Sun Tae Kim

LAB HEAD: Sun Tae Kim

PROVIDER: PXD057048 | Pride | 2025-04-23

REPOSITORIES: Pride

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			Action	DRS
	2nd-set_MSP1-YFP-rep_1.raw	Raw
	2nd-set_MSP1-YFP-rep_2.raw	Raw
	2nd-set_MSP1-YFP-rep_3.raw	Raw
	2nd-set_YFP-empty-rep_1.raw	Raw
	2nd-set_YFP-empty-rep_2.raw	Raw

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