Conversion of mouse fibroblast to hepatocyte-like cells by defined factors
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ABSTRACT: Plasticity of differentiated cells has been proved by nuclear transfer, induced pluripotent cells and transdifferentiation. Here we show that by transduction of 3 factors (Hnf1alpha, Gata4, and Foxa3) and p19Arf inactivation, tail-tip fibroblasts can be converted to hepatocyte-like (iHep) cells, expressing hepatocyte marker genes, and acquiring many mature hepatocyte functions in vitro and in vivo. p19Arf-null TTFs were tranfected with 3 liver enriched transcription factors, then changed to modified Block's medium. To enrich iHep cells, epithelial cells were enriched by partial trypsin digestion.
ORGANISM(S): Mus musculus
SUBMITTER: Lijian Hui
PROVIDER: E-GEOD-23635 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
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