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Direct reprogramming of human fibroblasts to functional hepatocyte-like cells

ABSTRACT: Plasticity of differentiated cells has been proved by nuclear transfer, induced pluripotent cells and transdifferentiation. Here we show that by transduction of 3 factors (FOXA3, HNF1A and HNF4A), human fetal fibroblasts can be converted to hepatocyte-like cells (hiHep cells), expressing hepatic marker genes, and acquiring many mature hepatocyte functions in vitro and in vivo. Human fetal fibroblasts (HFF) were tranfected with 3 liver enriched transcription factors (FOXA3, HNF1A, HNF4A), and converted to hepatocyte-like cells (hiHep cells). HFF and primary human hepatocytes (PHH) serve as control.

ORGANISM(S): Homo sapiens

SUBMITTER: Lijian Hui

PROVIDER: E-GEOD-42643 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Direct reprogramming of human fibroblasts to functional and expandable hepatocytes.

Huang Pengyu P Zhang Ludi L Gao Yimeng Y He Zhiying Z He Zhiying Z Yao Dan D Wu Zhitao Z Cen Jin J Chen Xiaotao X Liu Changcheng C Hu Yiping Y Lai Dongmei D Hu Zhenlei Z Chen Li L Zhang Ying Y Cheng Xin X Ma Xiaojun X Pan Guoyu G Wang Xin X Hui Lijian L

Cell stem cell 20140227 3

The generation of large numbers of functional human hepatocytes for cell-based approaches to liver disease is an important and unmet goal. Direct reprogramming of fibroblasts to hepatic lineages could offer a solution to this problem but so far has only been achieved with mouse cells. Here, we generated human induced hepatocytes (hiHeps) from fibroblasts by lentiviral expression of FOXA3, HNF1A, and HNF4A. hiHeps express hepatic gene programs, can be expanded in vitro, and display functions char ...[more]

PMID: 24582927

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Reprogramming fibroblasts into bi-potential hepatic stem cells by defined factors

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2013-07-19 | E-GEOD-48486 | biostudies-arrayexpress

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