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In Vitro Transcriptome Analysis of Porcine Plexus Epithelial Cells in Response to Streptococcus suis: Functions of the Choroid Plexus in Antimicrobial Defense

ABSTRACT: We used microarrays to detail the global gene expression changes following apical infection of porcine choroid plexus epithelial cells (PCPEC) with Streptococcus suis (S. suis) We compared PCPEC either un-treated or infected with wild-type S. suis strain 10 or the acapsular strain 10cpsDEF, respectively, to determine global gene expression changes by microarray analysis

ORGANISM(S): Sus scrofa

SUBMITTER: Ludger Klein-Hitpass

PROVIDER: E-GEOD-23751 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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In vitro transcriptome analysis of porcine choroid plexus epithelial cells in response to Streptococcus suis: release of pro-inflammatory cytokines and chemokines.

Schwerk Christian C Adam Rüdiger R Borkowski Julia J Schneider Henriette H Klenk Michael M Zink Sascha S Quednau Natascha N Schmidt Nicole N Stump Carolin C Sagar Anubha A Spellerberg Barbara B Tenenbaum Tobias T Koczan Dirk D Klein-Hitpass Ludger L Schroten Horst H

Microbes and infection 20110612 11

The Gram-positive zoonotic bacterium Streptococcus suis (S. suis) is responsible for a wide range of diseases including meningitis in pigs and humans. The blood-cerebrospinal fluid (CSF) barrier is constituted by the epithelial cells of the choroid plexus, which execute barrier function also after bacteria have entered the central nervous system (CNS). We show that the bacterial capsule, a major virulence factor, strongly attenuates adhesion of S. suis to the apical side of porcine choroid plexu ...[more]

PMID: 21683799

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In Vitro Transcriptome Analysis of Porcine Plexus Epithelial Cells in Response to Streptococcus suis: Functions of the Choroid Plexus in Antimicrobial Defense

Project description:We used microarrays to detail the global gene expression changes following apical infection of porcine choroid plexus epithelial cells (PCPEC) with Streptococcus suis (S. suis)

2011-08-15 | GSE23751 | GEO

ArgR is an essential local transcriptional regulator of the arcABC-operon in Streptococcus suis and crucial for biological fitness in acidic environment

Project description:Transcriptome comparison of Streptococcus suis wildtype and argR knock-out strain grown to exponential or early-stationary phase, respectively.

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Transcriptome comparison of Streptococcus suis wildytpe and a ccpA knock-out strain in exponential growth phase

Project description:Transcriptome comparison of Streptococcus suis wildytpe and a ccpA knock-out strain in exponential growth phase.

2010-12-31 | E-MEXP-2946 | biostudies-arrayexpress

Transcriptional immune response of PRRSV-infected porcine dendritic cells and monocytes to Streptococcus suis infection

Project description:We investigated for the first time the in vitro response of PRRSV-infected porcine DCs and monocytes to S. suis. We first assessed the effect of PRRSV infection on the phagocytosis and intracellular survival of S. suis by these two cell populations. We then used a genomic approach to compare the gene expression profiles of both type of cells infected with S. suis, with or without a previous infection with PRRSV. Total RNA obtained from porcine monocytes and dendritic cells infected with S. suis, PRRSV, or S. suis & PRRSV. Four replicates in all groups.

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Transcription profiling by array of cells starved of leucine in the presence or absence of a dominant negative HSF1 mutant (dnHSF1, HSF379).

Project description:Cells were starved for leucine in the presence or absence of a dominant negative HSF1 mutant (dnHSF1, HSF379). Changes in transcriptome relative to cells cultured with leucine were determined. The experiment shows the effect of dnHSF on the amino acid starvation response.

2012-12-11 | E-MEXP-3563 | biostudies-arrayexpress

Genome wide analysis of C57BL/6 mice and C57BL/6 TLR2-KO mice infected with European strain (P1/7) of Streptococcus suis

Project description:Streptococcus suis is an emerging zoonotic agent causing meningitis and septicemia. Outbreaks in humans in China with atypical cases of streptococcal toxic shock-like syndrome have been described to be caused by a clonal epidemic S. suis strain characterized as sequence type (ST) 7 by multilocus sequence typing, different from the classical ST1 usually isolated in Europe. Previous in vitro studies showed that Toll-like receptor (TLR) 2 plays a major role in S. suis ST1 interactions with host cells. In the present study, the in vivo role of TLR2 in systemic infections caused by S. suis ST1 or ST7 strains using TLR2 deficient (TLR2-/-) mice was evaluated. TLR2-mediated recognition significantly contributes to the acute disease caused by the highly virulent S. suis ST1 strain, since the TLR2-/- mice remained unaffected when compared to wild type (WT) mice. The lack of mortality could not be associated with a lower bacterial burden; however, a significant decrease in the induction of pro-inflammatory mediators, as evaluated by microarray, real-time PCR and protein assays, was observed. On the other hand, TLR2-/- mice infected with the epidemic ST7 strain presented no significant differences regarding survival and expression of pro-inflammatory mediators when compared to the WT mice. Together, these results show a TLR2-independent host innate immune response to S. suis that depends on the strain. Total RNA obtained from spleen of C57BL/6 mice or C57BL/6 TLR2-KO mice infected with Streptococcus suis strain P1/7. Four replicates in both groups.

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Genome wide analysis of C57BL-6 mice and C57BL/6 TLR2-KO mice infected with Chinese strain (SC84) of Streptococcus suis

2013-06-01 | E-GEOD-45862 | biostudies-arrayexpress

Transcriptional profiling of a flpS knock-out strain of Steptococcus suis strain 10

Project description:Transcriptional profiling of a S. suis 10flpS::spcR mutant strain in which the flpS gene has been disrupted by an insertion of a spectinomycin resistance cassette (spcR). We compared the expression profile of S. suis strain 10 with that of S. suis strain 10flpS::spcR during exponential and stationary growth of the bacterial cells.

2016-10-11 | E-MTAB-3997 | biostudies-arrayexpress

Genome wide analysis of C57BL-6 mice infected with North-American strain (89-1591) of Streptococcus suis

Project description:Streptococcus suis is a major swine pathogen that can be transmitted to humans causing severe symptoms. A large human outbreak was described in China, where approximately 25% out of 215 infected humans developed an unusual streptococcal toxic shock-like syndrome (STSLS). Albeit increased expression of inflammatory mediators following infection by the Chinese S. suis strain was suggested as responsible for STSLS case severity, the mechanisms involved are still poorly understood. In this study, we investigated the host innate immune response to infection by either one of 3 strains of S. suis: 89-1591 (Canadian, intermediate virulence), P1/7 (European, high virulence), and SC84 (Chinese, epidemic strain). Using Illumina microarray and validating those results with qPCR and Luminex assay, infected mice showed elevated expression of mainly pro-inflammatory chemokine and cytokine genes. Generally, pro-inflammatory genes were expressed at a higher level in mice infected with S. suis strain SC84 > P1/7 > 89-1591. Interestingly, IFNM-NM-3 was expressed at much higher levels only in mice infected with the S. suis strain SC84, which could potentially explain some of the STSLS symptoms. IFNM-NM-3-KO mice infected with SC84 showed better survival than WT mice while no differences was seen in mice infected with highly virulent P1/7 strain. Overall, our results show an important role of IFNM-NM-3 in S. suis infections and might explain in part the increased virulence of SC84 responsible for a recent outbreak in China. Total RNA obtained from spleen of C57BL/6 mice infected with Streptococcus suis strain 89-1591 or mock-infected with THB. Four replicates in both groups.

2013-03-27 | E-GEOD-41520 | biostudies-arrayexpress

Genome wide analysis of C57BL-6 mice infected with European strain (P1/7) of Streptococcus suis

Project description:Streptococcus suis is a major swine pathogen that can be transmitted to humans causing severe symptoms. A large human outbreak was described in China, where approximately 25% out of 215 infected humans developed an unusual streptococcal toxic shock-like syndrome (STSLS). Albeit increased expression of inflammatory mediators following infection by the Chinese S. suis strain was suggested as responsible for STSLS case severity, the mechanisms involved are still poorly understood. In this study, we investigated the host innate immune response to infection by either one of 3 strains of S. suis: 89-1591 (Canadian, intermediate virulence), P1/7 (European, high virulence), and SC84 (Chinese, epidemic strain). Using Illumina microarray and validating those results with qPCR and Luminex assay, infected mice showed elevated expression of mainly pro-inflammatory chemokine and cytokine genes. Generally, pro-inflammatory genes were expressed at a higher level in mice infected with S. suis strain SC84 > P1/7 > 89-1591. Interestingly, IFNM-NM-3 was expressed at much higher levels only in mice infected with the S. suis strain SC84, which could potentially explain some of the STSLS symptoms. IFNM-NM-3-KO mice infected with SC84 showed better survival than WT mice while no differences was seen in mice infected with highly virulent P1/7 strain. Overall, our results show an important role of IFNM-NM-3 in S. suis infections and might explain in part the increased virulence of SC84 responsible for a recent outbreak in China. Total RNA obtained from spleen of C57BL/6 mice infected with Streptococcus suis strain P1/7 or mock-infected with THB. Four replicates in both groups.

2013-03-27 | E-GEOD-41521 | biostudies-arrayexpress

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