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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling by array of cells starved of leucine in the presence or absence of a dominant negative HSF1 mutant (dnHSF1, HSF379).

ABSTRACT: Cells were starved for leucine in the presence or absence of a dominant negative HSF1 mutant (dnHSF1, HSF379). Changes in transcriptome relative to cells cultured with leucine were determined. The experiment shows the effect of dnHSF on the amino acid starvation response.

ORGANISM(S): Homo sapiens

SUBMITTER: Nicolette Lubsen

PROVIDER: E-MEXP-3563 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Heat shock factor 1 is inactivated by amino acid deprivation.

Hensen Sanne M M SM Heldens Lonneke L van Enckevort Chrissy M W CM van Genesen Siebe T ST Pruijn Ger J M GJ Lubsen Nicolette H NH

Cell stress & chaperones 20120714 6

Mammalian cells respond to a lack of amino acids by activating a transcriptional program with the transcription factor ATF4 as one of the main actors. When cells are faced with cytoplasmic proteotoxic stress, a quite different transcriptional response is mounted, the heat shock response, which is mediated by HSF1. Here, we show that amino acid deprivation results in the inactivation of HSF1. In amino acid deprived cells, active HSF1 loses its DNA binding activity as demonstrated by EMSA and ChIP ...[more]

PMID: 22797943

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