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Transcriptome analysis of the barley fast neutron mutants nec3

ABSTRACT: Affymetrix Barley1 GeneChip microarray was used to study the transcriptome of two fast neutron barley mutants FN362 and FN363 allelic to characterized necrotic leaf spot 3 (nec3) mutants. PCR screening of the 21 genes at least two-fold down-regulated in mutants did not show any deletions in the genome of mutants and, thus, failed to identify a gene responsible for the nec3 mutant phenotype suggesting that either probe set for the NEC3 gene is not on the Barley1 GeneChip, or that the expression of wt NEC3 is confined to specific developmental stage or tissue type. Transcriptome of the two barley fast neutron mutants was compared to the transcriptome of parental variety Steptoe

ORGANISM(S): Hordeum vulgare

SUBMITTER: Nils Rostoks

PROVIDER: E-GEOD-23775 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Transcriptome analysis of the barley fast neutron mutants nec3

Project description:Affymetrix Barley1 GeneChip microarray was used to study the transcriptome of two fast neutron barley mutants FN362 and FN363 allelic to characterized necrotic leaf spot 3 (nec3) mutants. PCR screening of the 21 genes at least two-fold down-regulated in mutants did not show any deletions in the genome of mutants and, thus, failed to identify a gene responsible for the nec3 mutant phenotype suggesting that either probe set for the NEC3 gene is not on the Barley1 GeneChip, or that the expression of wt NEC3 is confined to specific developmental stage or tissue type.

2010-08-25 | GSE23775 | GEO

Necrotic mutants in barley cv. Steptoe

Project description:FN044, FN211, FN242 and FN303 are the fast neutron generated mutants in cv. Steptoe background. These 4 mutants have lesion mimic phenotype and increase disease resistance to stem rust. ****[PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, ling zhang. The equivalent experiment is BB54 at PLEXdb.]

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Necrotic mutants in barley cv. Steptoe

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Soybean fast neutron genotyping: 700k Reverse genetics

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Heat stress expression data from developing barley caryopses

Project description:High temperature stress, like any abiotic stress, impairs the physiology and development of plants, including the stages of seed setting and ripening. In this study we used the 22K Barley1 GeneChip microarray to investigate the response of developing barley (Hordeum vulgare) caryopses at 12 days post anthesis to 0.5h, 3h and 6h of heat stress exposure. Developing barley caryopses were harvested at different time-points of a day during heat stress (42M-BM-0C) (2:00 PM, 5:00 PM and 8:00 PM). Heat stress started at 1:30PM. Pooled caryopses (form 3 plants) from the respective time points were used for RNA extraction and hybridization on Affymetrix microarrays (Barley1 GeneChip; GEO accsession GPL1340). Biological replicates were performed.

2010-11-22 | E-GEOD-23896 | biostudies-arrayexpress

Hordeum vulgare

Project description:Transcriptome analysis of the barley fast neutron mutants nec3

| PRJNA130625 | ENA

Soybean fast neutron genotyping: 700k Reverse genetics

Project description:CGH was used to compare induced structural variation among soybean fast neutron lines, developed in background M92-220.

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Soybean fast neutron genotyping: 700k Forward genetics

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Diurnal expression data from developing barley caryopses

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2010-04-07 | E-GEOD-20034 | biostudies-arrayexpress

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