Unknown,Transcriptomics,Genomics,Proteomics

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Representative Illumina sequencing libraries upon T7-polymerase based amplification

ABSTRACT: This experiment highlights the extreme sequence bias generated by standard PCR amplication of sequencing libraries and decribes an adapted T7-polymerase based amplification method, which results in non-baised, representative libraries for Illumina sequencing Adaptation of standard Illumina sequencing protocol to obtain representative sequencing libraries after sample amplification. Comparing different amplification methods with amplification free sequencing.

ORGANISM(S): Plasmodium falciparum 3D7

SUBMITTER: H. Stunnenberg 

PROVIDER: E-GEOD-23868 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

H2A.Z demarcates intergenic regions of the plasmodium falciparum epigenome that are dynamically marked by H3K9ac and H3K4me3.

Bártfai Richárd R   Hoeijmakers Wieteke A M WA   Salcedo-Amaya Adriana M AM   Smits Arne H AH   Janssen-Megens Eva E   Kaan Anita A   Treeck Moritz M   Gilberger Tim-Wolf TW   Françoijs Kees-Jan KJ   Stunnenberg Hendrik G HG  

PLoS pathogens 20101216 12

Epigenetic regulatory mechanisms and their enzymes are promising targets for malaria therapeutic intervention; however, the epigenetic component of gene expression in P. falciparum is poorly understood. Dynamic or stable association of epigenetic marks with genomic features provides important clues about their function and helps to understand how histone variants/modifications are used for indexing the Plasmodium epigenome. We describe a novel, linear amplification method for next-generation seq  ...[more]

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