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Representative Illumina sequencing libraries upon T7-polymerase based amplification

ABSTRACT: This experiment highlights the extreme sequence bias generated by standard PCR amplication of sequencing libraries and decribes an adapted T7-polymerase based amplification method, which results in non-baised, representative libraries for Illumina sequencing

ORGANISM(S): Plasmodium falciparum 3D7

PROVIDER: GSE23868 | GEO | 2010/10/28

SECONDARY ACCESSION(S): PRJNA133167

REPOSITORIES: GEO

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Representative Illumina sequencing libraries upon T7-polymerase based amplification

Project description:This experiment highlights the extreme sequence bias generated by standard PCR amplication of sequencing libraries and decribes an adapted T7-polymerase based amplification method, which results in non-baised, representative libraries for Illumina sequencing Adaptation of standard Illumina sequencing protocol to obtain representative sequencing libraries after sample amplification. Comparing different amplification methods with amplification free sequencing.

2010-10-28 | E-GEOD-23868 | biostudies-arrayexpress

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