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Expression analysis of Wv/Wv fetal gonads

ABSTRACT: Following sex determination, XY and XX gonads develop into a testis and an ovary, respectively. Depending on the sex of the gonad, resident germ cells will subsequently be committed to either spermatogenesis or oogenesis. In this study we took advantage of the Wv/Wv mouse genetic model, in which gonads are almost devoided of germ cells, to uncover gene expression underlying fetal germ cell development. Male and Female gonads were collected at 12, 14 and 16 days post coitum (dpc) from wild-type and Wv/Wv mouse embryos. Total RNAs were extracted and hybridized on Affymetrix microarrays. Expression signals from male and female gonads or from wild-type and mutant gonads were compared to identify sexually dimorphic genes as well as genes expressed in germ cell during fetal gonad development.

ORGANISM(S): Mus musculus

SUBMITTER: Antoine ROLLAND

PROVIDER: E-GEOD-23908 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Uncovering gene regulatory networks during mouse fetal germ cell development.

Rolland Antoine D AD Lehmann Kim P KP Johnson Kamin J KJ Gaido Kevin W KW Koopman Peter P

Biology of reproduction 20101208 4

The commitment of germ cells to either oogenesis or spermatogenesis occurs during fetal gonad development: germ cells enter meiosis or mitotic arrest, depending on whether they reside within an ovary or a testis, respectively. Despite the critical importance of this step for sexual reproduction, gene networks underlying germ cell development have remained only partially understood. Taking advantage of the W(v) mouse model, in which gonads lack germ cells, we conducted a microarray study to ident ...[more]

PMID: 21148109

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Expression analysis of Wv/Wv fetal gonads

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