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TopA mutant of P. aeruginosa PA14

ABSTRACT: We have tested the effect of deletion of Topoisomerase I on genes transcription of the bacteria Pseudomonas. aeruginosa. Topoisomerase I is a conserved protein that modulate the topology state of the DNA from super-coiled to relaxed. To this end we have compared the transcription profile of a topA::GentR mutant to that of the wild-type strain. The topA mutant contains in topA gene a Mariner transposon with a gentamicin resistance cassette. It is part of the non-redundant mutant library of P. aeruginosa PA14 strains. WT and mutant cells were grown to OD 2.0 in LB 200rpm shaking at 37C.

ORGANISM(S): Pseudomonas aeruginosa PA14

SUBMITTER: Laurence Rahme

PROVIDER: E-GEOD-24038 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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