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TopA mutant of P. aeruginosa PA14

ABSTRACT: We have tested the effect of deletion of Topoisomerase I on genes transcription of the bacteria Pseudomonas. aeruginosa. Topoisomerase I is a conserved protein that modulate the topology state of the DNA from super-coiled to relaxed. To this end we have compared the transcription profile of a topA::GentR mutant to that of the wild-type strain. The topA mutant contains in topA gene a Mariner transposon with a gentamicin resistance cassette. It is part of the non-redundant mutant library of P. aeruginosa PA14 strains.

ORGANISM(S): Pseudomonas aeruginosa PA14 Pseudomonas aeruginosa

PROVIDER: GSE24038 | GEO | 2011/12/21

SECONDARY ACCESSION(S): PRJNA130337

REPOSITORIES: GEO

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topA mutant of P. aeruginosa PA14

Project description:We have tested the effect of deletion of Topoisomerase I on genes transcription of the bacteria Pseudomonas. aeruginosa. Topoisomerase I is a conserved protein that modulate the topology state of the DNA from super-coiled to relaxed. To this end we have compared the transcription profile of a topA::GentR mutant to that of the wild-type strain. The topA mutant contains in topA gene a Mariner transposon with a gentamicin resistance cassette. It is part of the non-redundant mutant library of P. aeruginosa PA14 strains. WT and mutant cells were grown to OD 2.0 in LB 200rpm shaking at 37C.

2011-12-21 | E-GEOD-24038 | biostudies-arrayexpress

Pseudomonas aeruginosa PA14

Project description:topA mutant of P. aeruginosa PA14

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P. aeruginosa PA14-WT vs pyrF mutant vs pyrF mutant w/uracil

2008-11-12 | GSE9592 | GEO

The effect of 2-AA on P. aeruginosa PA14 and its mutants

Project description:The pqs operon of the bacteria P. aeruginosa is responsible for the production of at least 60 secreted compounds mainly hidroxy-quinolones (HAQs). Some of them, like PQS and HHQ, were found to be important signal that control the virulence of the bacteria. We have discover that one of the most abandon molecule produced by the pqs operon, 2 aminoacetophenone (2-AA), a volatile molecule serves as a unique signal. In contrast to the previous described PQS and HHQ, 2-AA is downregulating virulence and acute phase proteins and upregulating chronic phase proteins. In addition we have found that 2-AA promotes the formation of persisters cells that antibiotic tolerant. we have compared the genes profile of wild-type PA14 strain and its derivatives topA which lacks Topoisomerase I and pqsA that do not produces HAQs or 2-AA to cultures that were treated with 3mM of 2-AA. Cell were grown with and without 2-AA (3mM) to OD 2.0.

2011-12-21 | E-GEOD-24036 | biostudies-arrayexpress

P. aeruginosa PA14 mutant PA4496 biofilm vs wild-type biofilm

Project description:P. aeruginosa PA14 mutant strain PA4496 expression in biofilm cells relative to PA14 wild-type strain expression in biofilm cells. All samples cultured in LB with glass wool

2018-02-14 | GSE24638 | GEO

The effect of 2-AA on P. aeruginosa PA14 and its mutants

2011-12-21 | GSE24036 | GEO

Transcription profiling of P. aeruginosa PA14-WT vs pyrF mutant vs pyrF mutant w/uracil

Project description:Comparative transcriptome analyses of P. aeruginosa PA14 pyrF mutant with PA14 wild type, and with PA14 pyrF mutant with 1 mM uracil supplement and PA14 wild type with 10 mM uracil in biofilm cells. All samples were cultured in LB with glass wool at 37C for 7h. Experiment Overall Design: Strains: P. aeruginosa PA14 wild type, PA14 pyrF mutant Experiment Overall Design: Medium: LB for PA14 wild type and PA14 pyrF mutant, LB with 1 mM uracil for PA14 pyrF mutant, LB with 10 mM uracil for PA14 wild type Experiment Overall Design: Cell type: Biofilm cells grown on glass wool Experiment Overall Design: Time: 7 h Experiment Overall Design: Temperature: 37C

2008-11-14 | E-GEOD-9592 | biostudies-arrayexpress

Pseudomonas aeruginosa PA14 wild-type vs the tpbA (PA14_13660) mutant

Project description:Microarray analysis for the biofilm cells of Pseudomonas aeruginosa PA14 wild-type vs the tpbA (PA14_13660) mutant in LB medium at 4 and 7 h at 37C Microarray analysis for the biofilm cells of Pseudomonas aeruginosa PA14 wild-type vs the tpbA (PA14_13660) mutant in LB medium at 4 and 7 h at 37C.

2009-05-23 | E-GEOD-13871 | biostudies-arrayexpress

Pseudomonas aeruginosa PA14 wild-type vs the tpbA (PA14_13660) mutant

Project description:Microarray analysis for the biofilm cells of Pseudomonas aeruginosa PA14 wild-type vs the tpbA (PA14_13660) mutant in LB medium at 4 and 7 h at 37C

2009-05-23 | GSE13871 | GEO

Expression profile for higA mutant of Pseudomonas aeruginosa PA14

Project description:The previously uncharacterized proteins HigB (unannotated) and HigA (PA4674) of Pseudomonas aeruginosa PA14 were found to form a type II TA system in which antitoxin HigA masks the RNase activity of toxin HigB through direct binding. To determine the physiological role of HigB/HigA in P. aeruginosa, a whole-transcriptome experiment was performed for the higA antitoxin deletion mutant of the PA14 strain compared to the wild-type PA14 strain. The rationale was that for the strain that lacks the antitoxin, the effect of the toxin could be discerned due to enhanced activity of the toxin. Furthermore, toxin HigB reduces production of the virulence factors pyochelin, pyocyanin, swarming, and biofilm formation.

2016-11-04 | GSE74730 | GEO

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