Unknown,Transcriptomics,Genomics,Proteomics

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Genome-wide chromatin analysis of wing imaginal discs


ABSTRACT: We report the relationship between 'absent, small or homeotic discs 2' (ASH2) occupancy, histone modifications and the transcription machinery using the wing disc as a source of chromatin. Our results indicate that genes related to development and transcriptional regulation are direct targets of ASH2 and that this protein contributes to H3K4me3 of nearby nucleosomes. Moreover, ASH2 occupancy correlates with phosphorylated forms of RNA polymerase II and histone positive marks in active genes. RNA Pol II phosphorylation in Ser5 is reduced in ash2 mutants, indicating that ASH2 plays a role in promoter stalling. Examination of three histone modifications and two polymerase modifications in wing imaginal dics cells.

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Enrique Blanco 

PROVIDER: E-GEOD-24115 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Genome-wide chromatin occupancy analysis reveals a role for ASH2 in transcriptional pausing.

Pérez-Lluch Sílvia S   Blanco Enrique E   Carbonell Albert A   Raha Debasish D   Snyder Michael M   Serras Florenci F   Corominas Montserrat M  

Nucleic acids research 20110209 11


An important mechanism for gene regulation involves chromatin changes via histone modification. One such modification is histone H3 lysine 4 trimethylation (H3K4me3), which requires histone methyltranferase complexes (HMT) containing the trithorax-group (trxG) protein ASH2. Mutations in ash2 cause a variety of pattern formation defects in the Drosophila wing. We have identified genome-wide binding of ASH2 in wing imaginal discs using chromatin immunoprecipitation combined with sequencing (ChIP-S  ...[more]

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