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Genome-wide chromatin analysis of wing imaginal discs

ABSTRACT: We report the relationship between 'absent, small or homeotic discs 2' (ASH2) occupancy, histone modifications and the transcription machinery using the wing disc as a source of chromatin. Our results indicate that genes related to development and transcriptional regulation are direct targets of ASH2 and that this protein contributes to H3K4me3 of nearby nucleosomes. Moreover, ASH2 occupancy correlates with phosphorylated forms of RNA polymerase II and histone positive marks in active genes. RNA Pol II phosphorylation in Ser5 is reduced in ash2 mutants, indicating that ASH2 plays a role in promoter stalling.

ORGANISM(S): Drosophila melanogaster

PROVIDER: GSE24115 | GEO | 2011/02/17

SECONDARY ACCESSION(S): PRJNA130047

REPOSITORIES: GEO

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Genome-wide chromatin analysis of wing imaginal discs

Project description:We report the relationship between 'absent, small or homeotic discs 2' (ASH2) occupancy, histone modifications and the transcription machinery using the wing disc as a source of chromatin. Our results indicate that genes related to development and transcriptional regulation are direct targets of ASH2 and that this protein contributes to H3K4me3 of nearby nucleosomes. Moreover, ASH2 occupancy correlates with phosphorylated forms of RNA polymerase II and histone positive marks in active genes. RNA Pol II phosphorylation in Ser5 is reduced in ash2 mutants, indicating that ASH2 plays a role in promoter stalling. Examination of three histone modifications and two polymerase modifications in wing imaginal dics cells.

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Project description:Genotype: FRT82B Method: Staged wild type wing imaginal discs were dissected and transferred to DPBS. The wing imaginal discs were dissociated in 0.25% Trypsin-EDTA solution at 37 ℃ for 10 min. Cells were then washed in DPBS and passed through 35μm filter before library preparation. Construction of 10x single cell libraries and sequencing on Illumina Hiseq platform were performed by Novogene.

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