Regulation of Mammalian Cell Growth and Survival By an RNA Polymerase II-Pausing Factor
Ontology highlight
ABSTRACT:
ORGANISM(S): Mus musculus
SUBMITTER: Jianlong Sun
PROVIDER: E-GEOD-24180 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Publications
Genetic and genomic analyses of RNA polymerase II-pausing factor in regulation of mammalian transcription and cell growth.
Sun Jianlong J Pan Haihui H Lei Chengwei C Yuan Bin B Nair Sreejith J SJ April Craig C Parameswaran Balaji B Klotzle Brandy B Fan Jian-Bing JB Ruan Jianhua J Li Rong R
The Journal of biological chemistry 20110824 42
Many mammalian genes are occupied by paused RNA polymerase II (pol II) in the promoter-proximal region on both sides of the transcription start site. However, the impact of pol II pausing on gene expression and cell biology is not fully understood. In this study, we used a Cre-Lox system to conditionally knock out the b subunit of mouse negative elongation factor (Nelf-b), a key pol II-pausing factor, in mouse embryonic fibroblasts. We found that Nelf-b was associated with the promoter-proximal ...[more]
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