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Detection of aberrant DNA methylation in undifferentiated and differentiated human ES cells compared to normal fetal tissues


ABSTRACT: Embryonic stem cells can be differentiated in vitro to produce a variety of somatic cell types. We have employed the mDIP method combined with hybridization to a tiling microarray to obtain a genome-wide methylation analysis of all UnMethylated Regions(UMRs). We show that this differentiation is accompanied by an intrinsic process of extensive aberrant CpG island de novo methylation that includes developmental and cancer target genes. CpG-methylated genomic DNA was enriched using a methyl-DNA immunoprecipitation (mDIP) assay. DNA from the input and bound (enriched) DNA for each sample were labeled and hybridized on the array to define the methylation state of each region.

ORGANISM(S): Homo sapiens

SUBMITTER: Deborah Nejman 

PROVIDER: E-GEOD-24270 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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