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Expression data from differentiating ES cells expressing miR-200c and miR-141

ABSTRACT: ES cells express the miR-200 family which becomes down-regulated during the course of differentiation in serum. We generated an ES cell line which expresses miR-200c and miR-141 upon addition of doxycycline. Microarrays were used to gain a global picture of differentiation when miR-200c and miR-141 expression were maintained throughout differentiation through the addition of doxycycline. A2.miR200c ES cells, which express miR-200c/miR-141 upon addition of doxycycline, were differentiated as embryoid bodies in six petri dishes. Half of the samples were treated with doxycycline on days 2 and 4 of differentiation, and RNA was collected from all samples on days 3, 4, and 5.

ORGANISM(S): Mus musculus

SUBMITTER: Jennifer Gill

PROVIDER: E-GEOD-24289 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Expression data from differentiating ES cells expressing miR-200c and miR-141

Project description:ES cells express the miR-200 family which becomes down-regulated during the course of differentiation in serum. We generated an ES cell line which expresses miR-200c and miR-141 upon addition of doxycycline. Microarrays were used to gain a global picture of differentiation when miR-200c and miR-141 expression were maintained throughout differentiation through the addition of doxycycline.

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The Pitx2:miR-200c/141:noggin pathway regulates BMP signaling and ameloblast differentiation

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