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Mitochondrial mutations in cigarette smoke extract-chronically treated human oral OKF6 cells

ABSTRACT: The objective of this study was to analyze the mitochondrial mutations induced by chronic cigarette smoke extract treatment in human oral immortal OKF6 cells. The objective of this study was to analyze the mitochondrial mutations induced by chronic cigarette smoke extract treatment in human oral immortal OKF6 cells. 2 samples were included, a control passaged human oral immortal OKF6 cells and cigarette smoke extract-treated OKF6 cells. The chronic treatment for cigarette smoke extract is 7 months. Genomic DNAs were extracted from both cells and then used for Affymetix MitoChip Version 2.0 analysis.

ORGANISM(S): Homo sapiens

SUBMITTER: wenyue sun

PROVIDER: E-GEOD-24414 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Chronic CSE treatment induces the growth of normal oral keratinocytes via PDK2 upregulation, increased glycolysis and HIF1α stabilization.

Sun Wenyue W Chang Steven S SS Fu Yumei Y Liu Yan Y Califano Joseph A JA

PloS one 20110119 1

<h4>Background</h4>Exposure to cigarette smoke is a major risk factor for head and neck squamous cell carcinoma (HNSCC). We have previously established a chronic cigarette smoke extract (CSE)-treated human oral normal keratinocyte model, demonstrating an elevated frequency of mitochondrial mutations in CSE treated cells. Using this model we further characterized the mechanism by which chronic CSE treatment induces increased cellular proliferation.<h4>Methodology/principal findings</h4>We demonst ...[more]

PMID: 21283817

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Mitochondrial mutations in cigarette smoke extract-chronically treated human oral OKF6 cells

Project description:The objective of this study was to analyze the mitochondrial mutations induced by chronic cigarette smoke extract treatment in human oral immortal OKF6 cells. The objective of this study was to analyze the mitochondrial mutations induced by chronic cigarette smoke extract treatment in human oral immortal OKF6 cells.

2011-02-10 | GSE24414 | GEO

Cigarette smoke alters the secretome of lung epithelial cells

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2019-01-08 | PXD007180 | Pride

Human alveolar epithelial cells (A549) exposed to cigarette smoke extract

Project description:Human alveolar epithelial cells were exposed to cigarette smoke extract (CSE) for 1, 3 and 5 weeks at 1%, 5% and 10%, and gene expression was evaluated by complete transcriptome microarrays. In this study we explored the effect of cigarette smoke on the gene expression profile. Human alveolar epithelial cells stimulated with three different concentractions of CSE (1%, 5% and 10%) and for 1, 3 and 5 weeks were used for RNA extraction and hybridization on Affymetrix microarrays.

2016-02-16 | E-GEOD-77942 | biostudies-arrayexpress

Cigarette smoke extract-treated alveolar macrophage-derived extracellular vesicles

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Oxidative stress induces mitochondrial dysfunction and a protective unfolded protein response in RPE cells

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2014-01-30 | E-GEOD-49107 | biostudies-arrayexpress

The Effect of Repeated Whole Cigarette Smoke Challenge on Human Air-Liquid Interface Lung Epithelial Cultures

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2011-08-30 | E-GEOD-30660 | biostudies-arrayexpress

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Human airway epithelial responses to rhinovirus infection and cigarette smoke extract alone and in combination

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Proteomic analysis of vocal fold fibroblasts exposed to cigarette smoke extract

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2019-08-06 | PXD010640 | Pride

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